BQC BCA Protein Quantification Assay Kit is a simple test for the quantification of proteins in a wide variety of samples.
BQC BCA Protein Quantification Assay Kit is based on the bicinchoninic acid (BCA) assay. This method relies on two chemical reactions. The first is the Biuret reaction, in which Cu2+ is reduced to Cu+ by proteins in an alkaline solution. The second reaction is the chelation of reduced cupper ions by bicinchoninic acid to produce a purple complex with strong absorbance at 562 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard.