Cell-based assays for detecting Reactive Oxygen Species (ROS) are essential tools in studying oxidative stress, cellular damage, and redox biology. These assays typically use fluorescent probes that react with ROS inside live cells, allowing researchers to quantify and visualize oxidative activity in real time.
BQC probes are compatible with multiple detection platforms, including: Flow cytometry, Fluorescence microscopy and Fluorimetry.
Table. Comparison between BQC ROS Detection Cell based Assay Kits
| ROS Detection Cell based Assay kits | ||||
| DHE Probe | DCFH-Da Probe | DHR 123 | ||
| Reference | KP06002 | KP06003 | KP06004 | |
| Primary Target ROS | Superoxide (O₂⁻•) | General ROS (H₂O₂, OH•, ROO•, ONOO⁻) | Peroxynitrite, H₂O₂ (via peroxidases) | |
| Fluorescence | Ethidium (E+): ex 480 nm/em 576 nm 2-OH-E⁺: ex 500-530 nm/em 590-620 nm |
Dichlorofluorescein: ex 495 nm/em 529 nm | Rhodamine 123: ex 500 nm/em 536 nm | |
| Specificity | Moderate (multiple oxidation products) | Broad, less specific | Moderate to broad | |
