Total antioxidant capacity (TAC) is a global measurement of the non-enzymatic antioxidant defense system that integrates the individual effect of all antioxidants in a given matrix, and their additive, synergistic or antagonistic interactions. TAC is considered an important parameter to establish the redox state of a sample and can be used as an oxidative stress biomarker.
Due to the lack of standard quantification methods for TAC determination a wide variety of assays have been used to assess the antioxidant capacity of different types of samples. Most popular TAC assays are based on the spectrophotometric monitoring of reactions between antioxidants and specific oxidants or radicals generated in situ.
Table. Comparison between BQC Total Antioxidant Capacity (TAC) Assay Kits
| TAC Spectrophotometric assay kits | ||||||||||
| DMPD | ABTS | FRAP | ORAC | ORAC-B | CUPRAC | CUPRAC-B | Fast FRAP | DPPH | HORAC | |
| Reference | KF01001 | KF01002 | KF01003 | KF01004 | KF01004B | KF01005 | KF01005B | KF01006 | KF01007 | KF01008 |
| Time required for the assay | <30 min | <30 min | <15 min | 120 min | 120 min | <45 min | <45 min | <15 min | <15 min | 150 min |
| Detection method | Colorimetric (553 nm) |
Colorimetric (734 nm) |
Colorimetric (593 nm) |
Fluorimetric Kinetic (Ex= 485 nm; Em= 528-538 nm) |
Fluorimetric Kinetic (Ex= 485 nm; Em= 528-538 nm) |
Colorimetric (450 nm) |
Colorimetric (450 nm) |
Colorimetric (593 nm) |
Colorimetric (517 nm) |
Fluorimetric Kinetic (Ex= 480 nm; Em= 530 nm) |
| Analysis of substances | Hydrophilic and lipophilic | Hydrophilic and lipophilic | Hydrophilic | Hydrophilic | Lipophilic | Hydrophilic and lipophilic | Hydrophilic | Hydrophilic | Hydrophilic and lipophilic | Hydrophilic |
| Type of sample | Food, beverages and plant extracts | Biological samples, food and beverages | Biological samples, food and beverages | Biological samples, food and beverages | Lipophilic samples | Biological samples (with deproteinization step), food and beverages | Biological samples (without deproteinization) | Biological samples, food and beverages | Biological samples, food and beverages | Biological samples, food and beverages |
