Frequently Asked Questions
To provide instant access to a wide variety of information from our extensive product portfolio, BQC experts have created these technical resource and troubleshooting database to meet your needs.
Search by category to discover the possible cause of the problem and the recommended solution.
Sample erratic valuesadminBioq2022-10-18T13:38:28+02:00
Possible cause and recommended solutions:
- Samples contain interfering substances > Dilute sample further (if possible)
- Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use
- Samples not deproteinized > Use an appropriate deproteinization protocol
- Cells/Tissue samples not homogenized completely > Repeat the sample homogenization
- Inappropriate sample dilution buffer > Refer to Assay preparation
Dispersion of standard and sample readingsadminBioq2022-10-18T13:38:59+02:00
Possible cause and recommended solutions:
- Pipetting errors > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well
- Air bubbles formed in well(s) > Use reverse pipetting technique
Standard readings do not follow a linear patternadminBioq2022-10-18T13:40:07+02:00
Possible cause and recommended solutions:
- Pipetting errors in preparation of standards > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well
- Air bubbles formed in well(s) > Use reverse pipetting technique
- Standard stock is at incorrect concentration > Always refer to dilutions described in Assay preparation
- Improperly thawed reagents > Thaw all components completely and mix well before use
- Use of improperly stored reagents > Store the components appropriately and use fresh components from the standard curve
- Incorrect incubation times or temperatures > Refer to Assay protocol
Wells have color but there is no readingadminBioq2022-10-18T13:55:23+02:00
Possible cause and recommended solutions:
- Plate read at incorrect wavelength > Check the wavelength used in the assay
- Incorrect microplate > Use the correct microplate for your application:
- UV/Vis: transparent
- Fluorescence: black wells/transparent bottom
