BQC Lowry Protein Quantification Assay Kit is a simple test for the quantification of proteins in in different type of samples.
BQC Lowry Protein Quantification Assay Kit is based on the Lowry method. This method relies on two chemical reactions. The first is the Biuret reaction, in which protein reacts with cupric sulfate and tartrate in an alkaline solution, resulting in formation of copper-protein complexes. The second reaction is the reduction of the Folin-Ciocalteu Reagent (phosphomolybdate and phosphotungstate) by the previously formed chelated copper complexes producing a colored product that can be measured at 660 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard.
