BQC Bradford Protein Quantification Assay Kit is a simple and fast test for the quantification of proteins in a wide variety of samples.
The Bradford Protein Assay is a widely used colorimetric method for the rapid and accurate quantification of total protein in biological samples. Based on the binding of Coomassie Brilliant Blue G-250 dye to protein molecules, the assay produces a measurable color change from brown to blue with a maximum absorbance wavelength from 465 to 595 nm.
The amount of the dye-protein complex formed is directly related to a protein concentration and can be spectrophotometrically estimated by measuring the absorbance at 595 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard.
