BQC Protein Quantification Kits offer robust and reproducible solutions for measuring total protein concentration in a wide variety of biological samples. Designed for compatibility with standard lab workflows, these kits utilize proven methods such as BCA, Bradford, and Lowry assays. Proteins are biopolymeric structures composed of amino acids that play many critical roles in the body. Protein is also a vital part of the human diet. Protein quantification assays are therefore fundamental to biological research, clinical diagnosis or food industry.
Table. Comparison between BQC Protein Quantification Assay Kits
| Protein Quantification Spectrophotometric assay kits | ||||
| Bradford | Lowry | BCA | ||
| Reference | KB03003 | KB03004 | KB03005 | |
| Principle | Coomassie dye binding to protein | Copper ion reduction + Folin-Ciocalteu reaction | Copper ion reduction + BCA chelation | |
| Detection method | Colorimetric (595 nm) |
Colorimetric (660 nm) |
Colorimetric (562 nm) |
|
| Microplate linear range (BSA) | 50 – 1500 μg/mL Protein (high concentration) 1 – 30 μg/mL Protein (low concentration) |
50 – 800 μg/mL Protein | 200 – 1600 μg/mL Protein | |
| Cuvette linear range (BSA) | 1 – 30 μg/mL Protein | 1 – 30 μg/mL Protein | 200 – 1600 μg/mL Protein | |
| Assay time | < 15min | < 45min | < 30min | |
| Interferences | Sensitive to detergents and basic amino acids | Ammonium sulphate, detergents, chelating agents, reducing agents, salts, sugars, glycine and Tris buffer | Sensitive to reducing agents, chelators | |
| Best for | Quick checks, simple samples | Low concentration | General use, high-throughput, detergent-rich samples | |
