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info@bioquochem.com(+34) 985 26 92 92

KB03007 | Thiol Quantification Assay Kit

195.00455.00 TAX excl.

Thiol Quantification Assay Kit measures the reduced and oxidized thiol groups in biological samples using Ellman´s Reagent.
In the assay, Ellman´s Reagent, reacts with GSH (not GSSG), generating a colorimetric signal at 412 nm, so GSH can be specifically quantified.

In addition, the assay also allows the measurement of disulfides in the sample, thanks to NaBH4, which reduces them to thiols. GSSG is calculated by subtraction of GSH to GSH + GSSG.

Clear

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC and room temperature

Assay time: 30 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, and Standard (GSH)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Certificate of Analysis (CoA)

CoA_THIOL_30070311

CoA_THIOL_30071007

CoA_THIOL_30071101

CoA_THIOL_30071203

CoA_THIOL_30071304

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

  • Muñoz H, Fernández B, Perea M, et al. 2016. “e-BQC Electrochemical Antioxidant Capacity: Aging.” Free Radical Biology and Medicine 96: P-13. https://doi.org/10.1016/j.freeradbiomed.2016.04.076
  • Gonzalez P, Hevia D, Alonso R, et al. 2018. “GLUT1 protects prostate cancer cells from glucose deprivation-induced oxidative stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017.
  • Hevia D, Rey S, Riego M, Gomez E, and Muñoz H. 2018. “Chronic Heart Failure (CHF): Thiol Oxidation and Antioxidant Capacity as Possible Biomarker (e-BQC).” Free Radical Biology and Medicine 120: S85. https://doi.org/10.1016/j.freeradbiomed.2018.04.280

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