KB03007 | Thiol Quantification Assay Kit
205.00€ – 480.00€ TAX excl.
Thiol Quantification Assay Kit measures the reduced and oxidized thiol groups in biological samples using Ellman´s Reagent.
In the assay, Ellman´s Reagent, reacts with GSH (not GSSG), generating a colorimetric signal at 412 nm, so GSH can be specifically quantified.
In addition, the assay also allows the measurement of disulfides in the sample, thanks to NaBH4, which reduces them to thiols. GSSG is calculated by subtraction of GSH to GSH + GSSG.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: 4ºC and room temperature
Assay time: 30 minutes
Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, and Standard (GSH)
Necessary material: 96 well-plate spectrophotometer
If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com
Protocol Booklet
Download the web version
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Safety Data Sheet (SDS)
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Publications
- Muñoz H, Fernández B, Perea M, et al. 2016. “e-BQC Electrochemical Antioxidant Capacity: Aging.” Free Radical Biology and Medicine 96: P-13. https://doi.org/10.1016/j.freeradbiomed.2016.04.076
- Gonzalez P, Hevia D, Alonso R, et al. 2018. “GLUT1 protects prostate cancer cells from glucose deprivation-induced oxidative stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017.
- Hevia D, Rey S, Riego M, Gomez E, and Muñoz H. 2018. “Chronic Heart Failure (CHF): Thiol Oxidation and Antioxidant Capacity as Possible Biomarker (e-BQC).” Free Radical Biology and Medicine 120: S85. https://doi.org/10.1016/j.freeradbiomed.2018.04.280
FAQs
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