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KB03007 | Thiol Quantification Assay Kit

195.00455.00 TAX excl.

Thiol Quantification Assay Kit measures the reduced and oxidized thiol groups in biological samples using Ellman´s Reagent.
In the assay, Ellman´s Reagent, reacts with GSH (not GSSG), generating a colorimetric signal at 412 nm, so GSH can be specifically quantified.

In addition, the assay also allows the measurement of disulfides in the sample, thanks to NaBH4, which reduces them to thiols. GSSG is calculated by subtraction of GSH to GSH + GSSG.

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Additional information

Test

100, 200, 400

Publications

  • Gonzalez-Menendez, Pedro, David Hevia, Rebeca Alonso-Arias, Alejandro Alvarez-Artime, Aida Rodriguez-Garcia, Sandrina Kinet, Ivan Gonzalez-Pola, Naomi Taylor, Juan C. Mayo, and Rosa M. Sainz. 2018. “GLUT1 Protects Prostate Cancer Cells from Glucose Deprivation-Induced Oxidative Stress.” Redox Biology 17 (July): 112–27. https://doi.org/10.1016/j.redox.2018.03.017.
  • Hevia, David, Susana Rey, Marina Riego, Esther Gomez, and Henar Muñoz-Cimadevilla. 2018. “Chronic Heart Failure (CHF): Thiol Oxidation and Antioxidant Capacity as Possible Biomarker (e-BQC).” Free Radical Biology and Medicine 120: S85. https://doi.org/10.1016/j.freeradbiomed.2018.04.280
  • Muñoz-Cimadevilla, Henar, Beatriz Fernández, María Perea, Ana Puerta, and David Hevia. 2016. “E-BQC Electrochemical Antioxidant Capacity: Aging.” Free Radical Biology and Medicine 96: P-13. https://doi.org/10.1016/j.freeradbiomed.2016.04.076

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