KB03007 | Thiol Quantification Assay Kit

BQC Thiol Quantification Assay Kit is a selective, easy, and quick assay for measuring free thiols and disulfides groups in biological samples.

Protein (cysteine residues) and non-protein (e.g. homocysteine and glutathione) thiol groups (R-SH) are central components of the extracellular antioxidant defense. High concentrations of free thiols are indicative of a favorable redox status, while low levels of free thiols are associated with different oxidative stress related diseases. Reduced levels of free thiols arise from its rapid oxidation to disulfides in the presence of high amounts of reactive oxygen species (ROS).

BQC Thiol Quantification Kit is based on the Ellman’s method. The reaction between the Ellman’s Reagent (DTNB: 5,5’-dithiobis (2-nitrobenzoic acid)) and a thiol group (R-SH) leads to the formation of 2-nitro-5- thiobenzoate (TNB^–) that ionizes to the yellow colored (412 nm) dianion TNB^2- at alkaline pH. The intensity of the color produced is proportional to thiol concentration. In addition to free thiol determination, this kit also allows for the quantification of total thiols by reducing the disulfides groups present in a sample with NaBH₄.