KB03007 | Thiol Quantification Assay Kit

215.00503.00 TAX excl.

BQC Thiol Quantification Assay Kit is a selective, easy, and quick assay for measuring free thiols and disulfides groups in biological samples.

Protein (cysteine residues) and non-protein (e.g. homocysteine and glutathione) thiol groups (R-SH) are central components of the extracellular antioxidant defense. High concentrations of free thiols are indicative of a favorable redox status, while low levels of free thiols are associated with different oxidative stress related diseases. Reduced levels of free thiols arise from its rapid oxidation to disulfides in the presence of high amounts of reactive oxygen species (ROS).

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC and room temperature

Assay time: 30 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, and Standard (GSH)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

Download the web version

 

 

 

 

 

 

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Safety Data Sheet (SDS)

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Publications

  • Muñoz H, Fernández B, Perea M, et al. 2016. “e-BQC Electrochemical Antioxidant Capacity: Aging.” Free Radical Biology and Medicine 96: P-13. https://doi.org/10.1016/j.freeradbiomed.2016.04.076
  • Gonzalez P, Hevia D, Alonso R, et al. 2018. “GLUT1 protects prostate cancer cells from glucose deprivation-induced oxidative stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017.
  • Hevia D, Rey S, Riego M, Gomez E, and Muñoz H. 2018. “Chronic Heart Failure (CHF): Thiol Oxidation and Antioxidant Capacity as Possible Biomarker (e-BQC).” Free Radical Biology and Medicine 120: S85. https://doi.org/10.1016/j.freeradbiomed.2018.04.280

FAQs

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