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KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

240.00413.00 TAX excl.

Superoxide dismutases (SODs) are metalloenzymes that catalyze the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide. SOD Activity Assay Kit bases on the reduction of WST-1, which produces a water-soluble coloured formazan dye. The rate of the reduction with a superoxide anion linearly relates to the xanthine oxidase (XO) activity. By contrast, SOD inhibits this reaction. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Specifically, one unit of SOD corresponds to the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.

The conversion of xanthine and O2 to uric acid and H2O2 by XOD generates superoxide radicals. As a result, the superoxide anions reduce a tetrazolium salt (WST-1) to a colored formazan product (WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.

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Additional information

Test

100, 200, 400

Publications

  • Gila-Díaz, Andrea, Gloria Herranz Carrillo, Silvia Cañas, Miguel Saenz de Pipaón, José Antonio Martínez-Orgado, Pilar Rodríguez-Rodríguez, Ángel Luis López de Pablo, María A. Martin-Cabrejas, David Ramiro-Cortijo, and Silvia M. Arribas. 2020. “Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants during the First Month of Lactation.” Nutrients 12 (9): 1–14. https://doi.org/10.3390/nu12092569.
  • Gila-Díaz, A., Herranz Carrillo, G., Cañas, S., Saenz de Pipaón, M., Martínez-Orgado, J. A., Rodríguez-Rodríguez, P., … Arribas, S. M. (2020). Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation. Nutrients, 12(9), 2569
  • Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition

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