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KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

240.00413.00 TAX excl.

Superoxide dismutases (SODs) are metalloenzymes that catalyze the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide. SOD Activity Assay Kit bases on the reduction of WST-1, which produces a water-soluble coloured formazan dye. The rate of the reduction with a superoxide anion linearly relates to the xanthine oxidase (XO) activity. By contrast, SOD inhibits this reaction. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Specifically, one unit of SOD corresponds to the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.

The conversion of xanthine and O2 to uric acid and H2O2 by XOD generates superoxide radicals. As a result, the superoxide anions reduce a tetrazolium salt (WST-1) to a colored formazan product (WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.

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Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC

Assay time: 40 minutes

Reagents: Reagent A, Reagent B, Reagent C, and Reagent D

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Certificate of Analysis (CoA)

30111911_CoA_SOD

30112501_CoA_SOD

CoA_SOD_30110406

CoA_SOD_30110503

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

  • Gila A, Herranz G, Cañas S, et al. 2020. “Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation”. Nutrients, 12(9), 2569. https://doi.org/10.3390/nu12092569.
  • Phuthong S, Reyes CG, Rodríguez P, et al. 2020. “Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition” Int J Mol Sci. 22(1):237. doi: 10.3390/ijms22010237
  • Petrova P, Arsov A, Ivanov I, et al. 2021. “New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity” Microorganisms 9, 2127. https://doi.org/10.3390/microorganisms9102127
  • Sánchez A, Perelló M, Vélez EJ, et al. 2022. “Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.).” Antioxidants, 11, 319. https://doi.org/10.3390/antiox11020319

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