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KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

255.00435.00 TAX excl.

Superoxide dismutases (SOD) are metalloenzymes that catalyze the conversion (dismutation) of the superoxide anion (O2•−) into molecular oxygen (O2) and hydrogen peroxide (H2O2), playing a key role in the cellular antioxidant defense system.

BQC SOD Activity Assay Kit is based on the reduction of the water-soluble tetrazolium salt [WST-1, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt] to the water-soluble formazan dye (WST-1 formazan, λ = 450 nm) by superoxide radicals (O2•−).

In the presence of SOD, a decrease in the rate of WST-1 formazan generation is produced due to the dismutation of the superoxide radical (O2•−) catalyzed by the enzyme. Since the generation of WST-1 formazan is proportional to the amount of superoxide radical (O2•−), the SOD activity as an inhibition activity can be quantified by measuring the decrease in the formation of this compound at 450 nm.

SKU: KB03011Categories: Tags: , ,

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC

Assay time: 40 minutes

Reagents: Reagent A, Reagent B, Reagent C, and Reagent D

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at

Protocol Booklet

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  • Ramiro-Cortijo D, Gila-Diaz A, Herranz Carrillo G. 2022. “Influence of Neonatal Sex on Breast Milk Protein and Antioxidant Content in Spanish Women in the First Month of Lactation”. Antioxidants, 11(8), 1472. DOI: 10.3390/antiox11081472


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