KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

267.00456.00 TAX excl.

Superoxide dismutases (SOD) are metalloenzymes that catalyze the conversion (dismutation) of the superoxide anion (O2•−) into molecular oxygen (O2) and hydrogen peroxide (H2O2), playing a key role in the cellular antioxidant defense system.

BQC SOD Activity Assay Kit is based on the reduction of the water-soluble tetrazolium salt [WST-1, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt] to the water-soluble formazan dye (WST-1 formazan, λ = 450 nm) by superoxide radicals (O2•−).

In the presence of SOD, a decrease in the rate of WST-1 formazan generation is produced due to the dismutation of the superoxide radical (O2•−) catalyzed by the enzyme. Since the generation of WST-1 formazan is proportional to the amount of superoxide radical (O2•−), the SOD activity as an inhibition activity can be quantified by measuring the decrease in the formation of this compound at 450 nm.

SKU: KB03011Categories: Tags: , ,

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC

Assay time: 40 minutes

Reagents: Reagent A, Reagent B, Reagent C, and Reagent D

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

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Publications

  • Gila A, Herranz G, Cañas S, et al. 2020. “Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation”. Nutrients, 12(9), 2569. https://doi.org/10.3390/nu12092569
  • Phuthong S, Reyes CG, Rodríguez P, et al. 2020. “Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition” Int J Mol Sci. 22(1):237. doi: 10.3390/ijms22010237
  • Petrova P, Arsov A, Ivanov I, et al. 2021. “New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity” Microorganisms 9, 2127. https://doi.org/10.3390/microorganisms9102127
  • Sánchez A, Perelló M, Vélez EJ, et al. 2022. “Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.).” Antioxidants, 11, 319. https://doi.org/10.3390/antiox11020319
  • Ramiro-Cortijo D, Gila-Diaz A, Herranz Carrillo G. 2022. “Influence of Neonatal Sex on Breast Milk Protein and Antioxidant Content in Spanish Women in the First Month of Lactation”. Antioxidants, 11(8), 1472. DOI: 10.3390/antiox11081472
  • Sánchez A, Perelló M, Vélez EJ, et al. 2022. “Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.).” Antioxidants, 11, 319. DOI: 10.3390/antiox11020319
  • Ruvira, S.; Rodríguez-Rodríguez, P.; Ramiro-Cortijo, D.; Martín-Trueba, M.; Martín-Cabrejas, M.A.; Arribas, S.M. Cocoa Shell Extract Reduces Blood Pressure in Aged Hypertensive Rats via the Cardiovascular Upregulation of Endothelial Nitric Oxide Synthase and Nuclear Factor (Erythroid-Derived 2)-like 2 Protein Expression. Antioxidants 2023, 12, 1698. https://doi.org/10.3390/antiox12091698
  • Cañas, S.; Rebollo-Hernanz, M.; Bermúdez-Gómez, P.; Rodríguez-Rodríguez, P.; Braojos, C.; Gil-Ramírez, A.; Benítez, V.; Aguilera, Y.; Martín-Cabrejas, M.A. Radical Scavenging and Cellular Antioxidant Activity of the Cocoa Shell Phenolic Compounds after Simulated Digestion. Antioxidants 2023, 12, 1007. https://doi.org/10.3390/antiox12051007

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