Galic Acid Standard
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Galic Acid Standard
To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples
Description
Galic Acid Standard
INTRODUCTION
Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples.
RECOMMENDED USES
To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples.
COMPATIBLE BUFFERS
| Reference | Product | Description |
| ST08006 | Bio-Buffer | 150 mM Sterile PBS pH 7.4 |
| ST08007 | General Buffer | 100 Mm PBS pH 7 |
| ST08005 | Nutrition Buffer | 0.1 M PBS pH 5.8 |
STANDARD CALIBRATION
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
| Standard (µl) | Diluent (µl) | GAE (µM) | |
| 1 | 0 | 1000 | 0 |
| 2 | 10 | 990 | 10 |
| 3 | 25 | 975 | 25 |
| 4 | 50 | 950 | 50 |
| 5 | 75 | 925 | 75 |
| 6 | 100 | 900 | 100 |
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
e-BQC SPECIFICITY
Gallic acid is more predominantly present in Q2 where it has higher sensitivity. It is recommended to use Q2 for calibrations at low concentrations of Gallic acid.
| Q1 | Q2 | Qt | |
| Sensitivity | 0.02 µC/ µM | 0.05 µC/ µM | 0.8 µC/ µM |
| LOD | 5 µM | 10 µM | 7 µM |
| LOQ | 17 µM | 30 µM | 25 µM |
| LOB | 2 µM | 12 µM | 14 µM |
| RSD | 15 % | 10 % | 14 % |
Instructions
Download ST08002 manual