Periodic Acid Schiff (PAS) – Mayer’s Hematoxylin | KH07011

Description

Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Mayer’s hematoxylin is used to stain cell nuclei.

Uses and sample preparation:

Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

Components

• Schiff Reagent
• Periodic acid 0,5%
• Sulfurose Acid Reagent
• Mayer’s Hematoxylin

Short Procedure

1. Deparaffinize and hydrate
2. Add periodic acid 0,5%
3. Rinse with distilled water
4. Add Schiff Reagent
5. Rinse with 3 baths of sulfurose acid
6. Rinse with tap water
7. Place in hematoxylin
8. Rinse with tap water
9. Dehydrate, clear and coverslip

Staining characteristics:

Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple