COMPARISON AMONG REDOX FLUORESCENT PROBES

Redox probes are sometimes considered to be equivalent and to react with all free radicals equally, so choosing one may seem tricky. However, they tend to be more reactive towards one than others and so the suitability should be considered depending on the aim of the experiments and the samples to be tested. In this blog post, we are going to show the differences, similarities, reactiveness and recommendations for each probe from our catalogue, hoping to shed some light into this topic:

DHE, DCFH-DA and DHR-123

Reacts directly with:

  • O2
  • H2O2 in the presence of Fe2+ or cytocrome c

Interferences:

  • 2-OH-E+

Recommendations:

Suitable for detection of intracellular oxidants However, red fluorescence measurements using fluorescence or confocal microscopy will not be able to distinguish between E+ and 2-OH-E+.

Measurement of H2O2 in the presence of Fe2+ or cytochrome c; and O2

Reacts directly with:

  • OH
  • Compounds I and II
  • NO2 formed from the myeloperoxidase/ H2O2/NO2 system
  • Hypoclorus acid (HOCl)
  • Reactive species formed form peroxynitrite (ONOO/ONOOH) decomposition
  • H2O2 in the presence of Fe2+

Reacts indirectly with:

  • H2O2

Interferences:

  • Heme proteins: hemoglobin, cytochrome c released during apoptosis
  • O2 : Artifactual amplification of the fluorescence signal intensity

Recommendations:

Suitable as a redox indicator probe for uncovering new redox signaling mechanisms keeping in mind the various caveats.

Measurement of H2O2, ROO· and ONOO

Reacts directly with:

  • Cogenerated O2and NO
  • OH
  • Compounds I and II
  • H2O2 in the presence of Fe2+, peroxidase or cytochrome c
  • NO2 formed from the myeloperoxidase/ H2O2/NO2 system
  • Reactive species formed form peroxynitrite (ONOO/ONOOH) decomposition
  • Hypoclorus acid (HOCl)

Reacts indirectly with:

  • ONOO
  • O2

Interferences:

  • O2: Artifactual amplification of the fluorescence signal intensity

Recommendations:

Suitable as a nonspecific indicator for intracellular ONOO or oxidants derived from it.

Measurement of H2O2, HOCl and ONOO

Multiprobe

Are you still undecided?

We have a product for you! With the Multiprobe redox kit, you will have the opportunity to test all of them so you will see which one fits your necessities best.