Protein Quantification and Western Blot

Protein Quantification

BQCkit’s Protein Quantification Line provides different techniques for protein quantification in biological samples.

These kits can be based on the copper reaction (Lowry ¹ and BCA ²) or in a colorimetric dye (Bradford ³).

  • Bradford Protein Quantification Assay Kit

    KB03003 | Bradford Protein Quantification Assay Kit

    104,00179,00 TAX excl.
  • Lowry Protein Assay- Protein Quantification | BQCkit

    KB03004 | Lowry Protein Assay- Protein Quantification | BQCkit

    143,00 TAX excl.
  • BCA Protein Assay Kit - BQCkit

    KB03005 | BCA Protein Assay Kit | BQCkit

    135,00215,00 TAX excl.

Western Blot

Western Blot (or protein immunoblot) is one of the most used techniques in molecular biology to detect proteins.

BQCkits ECL Substrate and Ponceau Solution provide you high-quality reagents to help you achieve the optimal results in this technique.

  • ECL Western Blotting Detection Reagents-BQCkit

    KB03020 | ECL Western Blotting Detection Reagents-BQCkit

    149,00223,00 TAX excl.
  • Ponceau S-Staining Solution-BQCkit

    SP04001 | Ponceau S-Staining Solution | BQCkit

    62,00 TAX excl.

¹ Method for protein quantification based on the reaction of cooper ions. It’s normally used with protein concentrations between 0.01–1.0 mg/mL.

² It’s one of the most popular methods for quantitation of total protein with colorimetric detection. Its popularity come from its compatibility with samples that contain up to 5% surfactants.

³ Method of spectroscopic analytical used to measure the concentration of protein in a solution.

This method is used for detect proteins which are bond indirectly to the secondary antibody HRP.

This compound is a sodium salt of a diazo dye caracterized by a light red color. It’s used on Western Blot technique for detection of protein bands on nitrocellulose or polyvinylidene fluoride.