Protein Quantification and Western Blot

Protein Quantification

BQCkit’s Protein Quantification Line provides different techniques for protein quantification in biological samples.

These kits can be based on the copper reaction (Lowry ¹ and BCA ²) or in a colorimetric dye (Bradford ³).

KB03003 | Bradford Protein Quantification Assay Kit
104,00€ – 179,00€ TAX excl.

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KB03004 | Lowry Protein Assay- Protein Quantification | BQCkit
143,00€ TAX excl.

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KB03005 | BCA Protein Assay Kit | BQCkit
135,00€ – 215,00€ TAX excl.

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Western Blot

Western Blot (or protein immunoblot) is one of the most used techniques in molecular biology to detect proteins.

BQCkits ECL Substrate ⁴ and Ponceau Solution ⁵ provide you high-quality reagents to help you achieve the optimal results in this technique.

¹ Method for protein quantification based on the reaction of cooper ions. It’s normally used with protein concentrations between 0.01–1.0 mg/mL.

² It’s one of the most popular methods for quantitation of total protein with colorimetric detection. Its popularity come from its compatibility with samples that contain up to 5% surfactants.

³ Method of spectroscopic analytical used to measure the concentration of protein in a solution.

⁴ This method is used for detect proteins which are bond indirectly to the secondary antibody HRP.

⁵ This compound is a sodium salt of a diazo dye caracterized by a light red color. It’s used on Western Blot technique for detection of protein bands on nitrocellulose or polyvinylidene fluoride.

Protein Quantification and Western Blot