Oxidative Stress Line

Antioxidant Capacity

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.

BQCkit’s Antioxidant Capacity Assays are useful for the determination of this parameter in different sample types such as food and beverages or plasma.

There are two types of mechanisms to determine the antioxidant capacity on different matrices: electron-transfer (E-T) methods, which measure the ability of an antioxidant to reduce an oxidant, with a change of color (ABTS, FRAP, DMPD) and hydrogen atom mediated (HAT) methods, which measure the ability of an antioxidant to eliminate free radicals through the donation of a hydrogen atom (they normally quantify peroxyl radicals; ORAC).

A comparison between BQCkit Antioxidant Capacity products is in the table below.

Comparison between BQCkit Antioxidant Capacity Products


DMPDABTSFRAPORACCUPRACDPPH
KF01001 KF01002 KF01003 KF01004 KF01005DPPH
Detection method colorimetric (553 nm) colorimetric (734 nm)colorimetric (593 nm)fluorimetric (Ex= 485 nm; Em= 528-538 nm)colorimetric (450 nm)colorimetric (517 nm)
Reaction pH acid acid acid physiological physiological
Analysis of hydrophilic/lipophilic substances hydrophilichydrophilic and lipophilic hydrophilic hydrophilic and lipophilichydrophilic and lipophilic hydrophilic and lipophilic
Type of sample food and beverages food and beveragesbiological samples (plasma)biological samples, cell lysates, tissue homogenates, food and beveragesbiological samples, food and beveragesFood and beverages
Type of measurement endpoint endpoint endpoint kinetic kinetic endpoint

Enzymatic Assays

Superoxide dismutases (SODs) are enzymes that catalyze the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide.

BQCkit’s SOD Activity Assay kit is recommended to quantified this reaction by the reduction of WST-1. Additionally, hydrogen peroxide is destructed by catalase by dismutation as a defense mechanism. Actually, it is related to antioxidant capacity.

BQCkit’s Catalase Activity Assay consists of a colorimetric reaction, which is proportional to this enzymatic activity.

Biomolecule Assays

Reactive Oxygen Species (ROS) is a product of normal aerobic metabolism and can damage cells, by oxidizing DNA, lipids and proteins.

BQCkit offers a wide range of products to quantify lipid peroxidation, thiols, protein carbonylation, nitrite and nitrate.

ROS Probes

Fluorescent REDOX probes are permeant fluorogenic dyes designed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy of fluorimeter.

Bioquochem under its brand BQCkit offers a variaty of Intracellular ROS Assays to help with your research.

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