TROLOX Standard

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TROLOX Standard

To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC).

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SKU: ST08003 Category: Tags: , ,

Description

TROLOX Standard

INTRODUCTION

Trolox is a water soluble analog of tocopherol (Vitamin E) which is a very strong antioxidant, commonly used to measure antioxidant capacity. Vitamin E is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples.

COMPATIBLE BUFFERS

ProductReference
150 mM Sterile PBS pH 7.4ST08006

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

 Standard (µl)Diluent (µl)TEAC (µM)
1010000
25095050
3100900100
4200800200
5400600400
6600400600
7800200800
8100001000

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

TROLOX is more predominantly present in Q1 where it has lower detection limits and less interferences. It is recommended to use Q1 for calibrations at low concentrations of TROLOX.

 Q1Q2Qt
Sensitivity0.05 µC/ µM0.05 µC/ µM0.11 µC/ µM
LOD2 µM90 µM42 µM
LOQ7 µM300 µM140 µM
LOB0.8 µM7 µM8 µM
RSD15 %13 %14 %

 

COMPARISON WITH TAC ASSAY KITS

If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an TROLOX standard could be used:

ProductReference
DMPDKF01001
FRAPKF01003
FAST FRAPKF01006
ORACKF01004

 

Additional information

Vol

5 ml