TROLOX Standard | ST08003

36,00 TAX excl.

TROLOX Standard

To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC).

Clear
SKU: ST08003 Category: Tags: , ,

Description

TROLOX Standard

INTRODUCTION

Trolox is a water soluble analog of tocopherol (Vitamin E) which is a very strong antioxidant, commonly used to measure antioxidant capacity. Vitamin E is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples.

COMPATIBLE BUFFERS

Product

Reference

150 mM Sterile PBS pH 7.4

ST08006

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

 

Standard (µl)

Diluent (µl)

TEAC (µM)

1

0

1000

0

2

50

950

50

3

100

900

100

4

200

800

200

5

400

600

400

6

600

400

600

7

800

200

800

8

1000

0

1000

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

TROLOX is more predominantly present in Q1 where it has lower detection limits and less interferences. It is recommended to use Q1 for calibrations at low concentrations of TROLOX.

 

Q1

Q2

Qt

Sensitivity

0.05 µC/ µM

0.05 µC/ µM

0.11 µC/ µM

LOD

2 µM

90 µM

42 µM

LOQ

7 µM

300 µM

140 µM

LOB

0.8 µM

7 µM

8 µM

RSD

15 %

13 %

14 %

 

COMPARISON WITH TAC ASSAY KITS

If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an TROLOX standard could be used:

Product

Reference

DMPD

KF01001

FRAP

KF01003

FAST FRAP

KF01006

ORAC

KF01004

 

Additional information

vol

5 ml

[contact-form-7 id=”6630″ title=”Form for quotation”]