Polyphenol Quantification Assay Kit
Polyphenol quantification kit by the Folin-Ciocalteu (FC) method is convenient and simple. In addittion, it requires only common equipment and has produced a large body of comparable data. Under proper conditions, the assay can also quantify monophenols and gives predictable reactions with the types of phenols found in nature. Because different phenols react to different degrees, expression of the results as a single number—such as milligrams per liter gallic acid equivalence—is necessarily arbitrary.
The F-C assay has been used as a measure of total phenols in natural products. Its basic mechanism is an oxidation/reduction reaction. The original F-C assay, used the carbonate buffer for pH adjustment. Addittionally, the end-point of the reaction after 120 min at room temperature, which makes difficult its implementation for routine analysis .
The method proposed by Bioquochem can be performed both in a 96-well microplate format or in a cuvette. Therefore, it can be applied to several phenolic compounds and food products (wines, beers, infusions, juices).
Polyphenols are a structural class of mainly natural, but also synthetic or semisynthetic organic chemicals. They are characterized by the presence of large multiples of phenol structural units. The number and characteristics of these phenol structures underlie the unique physical, chemical, and biological (metabolic, toxic, therapeutic, etc.) properties of particular members of the class. These compounds may be classified into different groups. In depends on the number of phenol rings that they contain and the structural elements that bind these rings to one another. Distinctions are thus made between the phenolic acids, flavonoids, stilbenes, and lignans .
From the nutritional point of view, Polyphenols are abundant micronutrients in our diet. Evidence for their role in the prevention of degenerative diseases such as cancer and cardiovascular diseases is emerging. The health effects of polyphenols depend on the amount consumed and on their bioavailability. In addition to having antioxidant properties, polyphenols have several other specific biological actions that are as yet poorly understood.