ORAC Assay – Oxygen Radical Antioxidant Capacity‎ | KF01004

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The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein, to result in a change of fluorescent intensity and the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.

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Description

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Antioxidants are substances with antioxidative properties. Antioxidants are most commonly present in fruits and vegetables. In addittion, other foods such as wine, meat or eggs contain these substances.

Bioquochem’s ORAC Activity Assay

The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein. As a result, there is a change of fluorescent intensity. Hence, the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.
It is possible to quantify the protection by calculating the area under the curve (AUC) from the experimental sample. After subtracting the AUC for the blank, the resultant difference would be the protection that the antioxidant compound confers. Trolox® (6-hydroxy-2,5,7,8-tetrametmethylchroman-2-carboxylic acid), a water-soluble vitamin E analog, is used as the calibration standard and ORAC results are expressed as Trolox® equivalents.

Check our blog post about ORAC Data Analysis 

Additional information

Test

192 test (96 well plate), 96 test (96 well plate), 960 test (96 well plate)

Manual

Download KF01004 manual