Galic Acid Standard
Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples.
To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples.
|ST08006||Bio-Buffer||150 mM Sterile PBS pH 7.4|
|ST08007||General Buffer||100 Mm PBS pH 7|
|ST08005||Nutrition Buffer||0.1 M PBS pH 5.8|
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
Gallic acid is more predominantly present in Q2 where it has higher sensitivity. It is recommended to use Q2 for calibrations at low concentrations of Gallic acid.
0.02 µC/ µM
0.05 µC/ µM
0.8 µC/ µM
COMPARISON WITH TAC ASSAY KITS
If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with a TROLOX standard could be used: