DHR-123 (Dihydrorhodamine) | KP06004

147,00295,00 TAX excl.

Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).

Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively.

It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).

DHR 123 probe is a READY TO USE probe suitable for:

Flow cytometer

Microscopy

Fluorimeter

 

Clear

Description

Dihydrorhodamine 123 probe (Intracellular ROS assay) Principle

Some stress conditions like exposure to oxidants or drugs can induce Reactive Oxygen Species (ROS). As a result, ROS induce damage in DNA, protein and lipids, with important effects in cells, leading to oxidative stress.

Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is an uncharged and nonfluorescent reactive oxygen species (ROS) indicator that can passively diffuse across membranes. Oxidation to cationic rhodamine 123 occurs inside cell, which specifically localizes in the mitochondria and exhibits green fluorescence.

It seems that neither the superoxide, the NO, nor the hydrogen peroxide by themselves, are capable of oxidizing DHR 123. According to literature, these ROS, combine with other cellular components such as cytochrome c oxidase or Fe2+ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123.

This probe is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Then, after cell uptake, ROS oxidize DHR 123 into a fluorescent compound.

BQCkit Dihydrorodamine 123 probe presents a solution 1000 times concentrated of DHR 123 ready to use. The protocol for this probe is very simple and contains a stain step, an incubation step and a “semiwash” step. The user can monitor Formation of Rhodamine 123 by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm.

There are three different formats according to the customer needs. These formats are: 250, 500 and 1000 tests.

 

 

Additional information

Test

1000 test, 250 test, 500 test

Color

Blue, Yellow, Red, Pink, White