Caspase-9 Colorimetric Assay Kit | KC04011

268,00535,00 TAX excl.

Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes that play essential roles in programmed cell death. Caspase activity is measured by a short peptide containing specific cleavage sequences that are recognized by the caspase and are covalently attached to a colorimetric or fluorogenic detection probe. Initiator caspases (caspase 2, 8, 9 and 10) initiate the apoptosis signal and the executioner caspases (caspase 3, 6, and 7) carry out the mass proteolysis that leads to apoptosis.

Bioquochem’s Caspase-9 Colorimetric Assay Kit bases in a short peptide that contains specific cleavage sequences recognized by the caspase. As a result, caspase-9 cleaves the reagent and releases the colorimetric or fluorogenic detection probe.

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Description

Bioquochem’s Caspase-9 Colorimetric Assay Kit Principle

Bioquochem’s Caspase-9 Colorimetric Assay Kit provides a simple procedure for assaying the activity of caspases that recognize the sequence LEHD. Hence, the user can detect the chromophore p-nitroanilide (pNA), after cleavage from the labeled substrate LEHD-pNA. The pNA can be quantified using a spectrophotometer or a microplate reader at 405 nm.  Comparison pNA absorvance from an apoptotic sample vs absorvance of control allows determination of the fold increase in caspase-9 activity

Information

Caspases are also known as cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases. They are a family of protease enzymes that play essential roles in programmed cell death. Caspases have the ability to cleave after aspartic acid residues.

There are two classes of caspases that involve in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. As a consequence, activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. As a resume, initiator caspases (caspase 2, 8, 9 and 10) initiate the apoptosis signal and the executioner caspases (caspase 3, 6, and 7) carry out the mass proteolysis that leads to apoptosis.

Caspase 9 is also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)). Specifically,iIt is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has two isoforms, according to literature. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 takes part in the caspase activation cascade, responsible for apoptosis execution, and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.

Bioquochem’s Caspase-9 Colorimetric Assay Kit bases in a short peptide that contains specific cleavage sequences recognized by the caspase. As a result, caspase-9 cleaves the reagent and releases the colorimetric or fluorogenic detection probe.

 

Additional information

Test

100 test (96 well plate), 200 test (96 well plate), 500 test (96 well plate)

Manual

Download KC04011 manual