Description
Ascorbic Acid Standard
INTRODUCTION
Ascorbic acid or Vitamin C is the strongest natural water-soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive.
RECOMMENDED USES
To compare the e-BQC results (µC) to classical antioxidant capacity units of Vitamin C Antioxidant Capacity Equivalents (CEAC). It could be used for both biological and food samples.
COMPATIBLE BUFFERS
Product | Reference |
0.1 M PBS pH 7 | |
150 mM Sterile PBS pH 7.4 | |
0.1 M PBS pH 5.8 |
STANDARD CALIBRATION
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
| Standard (µl) | Diluent (µl) | CEAC (µM) |
1 | 0 | 1000 | 0 |
2 | 50 | 950 | 50 |
3 | 100 | 900 | 100 |
4 | 200 | 800 | 200 |
5 | 400 | 600 | 400 |
6 | 600 | 400 | 600 |
7 | 800 | 200 | 800 |
8 | 1000 | 0 | 1000 |
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
e-BQC SPECIFICITY
Ascorbic acid is more predominantly present in Q1 where it presents lower detection limits and less interferences. It is recommended to use Q1 for calibrations at low concentrations of ascorbic acid.
| Q1 | Q2 | Qt |
Sensitivity | 0.07 µC/ µM | 0.04 µC/ µM | 0.10 µC/ µM |
LOD | 2 µM | 10 µM | 12 µM |
LOQ | 3 µM | 16 µM | 19 µM |
LOB | 1.7 µM | 9.2 µM | 10.9 µM |
RSD | 8 % | 5 % | 5 % |
COMPARISON WITH TAC ASSAY KITS
If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an ascorbic acid standard could be used:
Product | Reference |
KF01002 | |
KF01003 | |
KF01006 | |
KF01004 |
