Ascorbic Acid Standard

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Ascorbic Acid Standard

Ascorbic acid or Vitamin C is the strongest natural water-soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive. Bioquochem offers Ascorbic Acid Standard for its use with the e-BQC device.

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SKU: ST08001 Category: Tags: , ,

Ascorbic Acid Standard

INTRODUCTION

Ascorbic acid or Vitamin C is the strongest natural water-soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive.

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of Vitamin C Antioxidant Capacity Equivalents (CEAC). It could be used for both biological and food samples.

COMPATIBLE BUFFERS

ProductReference
0.1 M PBS pH 7ST08007
150 mM Sterile PBS pH 7.4ST08006
0.1 M PBS pH 5.8ST08005

 

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

 Standard (µl)Diluent (µl)CEAC (µM)
1010000
25095050
3100900100
4200800200
5400600400
6600400600
7800200800
8100001000

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknown. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

Ascorbic acid is more predominantly present in Q1 where it presents lower detection limits and fewer interferences. It is recommended to use Q1 for calibrations at low concentrations of ascorbic acid.

 Q1Q2Qt
Sensitivity0.07 µC/ µM0.04 µC/ µM0.10 µC/ µM
LOD2 µM10 µM12 µM
LOQ3 µM16 µM19 µM
LOB1.7 µM9.2 µM10.9 µM
RSD8 %5 %5 %

 

COMPARISON WITH TAC ASSAY KITS

If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an ascorbic acid standard could be used:

ProductReference
ABTSKF01002
FRAPKF01003
FAST FRAPKF01006

 

Instructions

Download ST08001 manual