Ascorbic Acid Standard | ST08001

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Ascorbic Acid Standard

Ascorbic acid or Vitamin C is the strongest natural water-soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive. Bioquochem offers Ascorbic Acid Standard for its use with the e-BQC device. 

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SKU: ST08008-1 Category: Tags: , ,

Description

Ascorbic Acid Standard

INTRODUCTION

Ascorbic acid or Vitamin C is the strongest natural water-soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive.

RECOMMENDED USES

To compare the e-BQC results (µC) to classical antioxidant capacity units of Vitamin C Antioxidant Capacity Equivalents (CEAC). It could be used for both biological and food samples.

COMPATIBLE BUFFERS

Product

Reference

0.1 M PBS pH 7

ST08007

150 mM Sterile PBS pH 7.4

ST08006

0.1 M PBS pH 5.8

ST08005

 

STANDARD CALIBRATION

Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:

 

Standard (µl)

Diluent (µl)

CEAC (µM)

1

0

1000

0

2

50

950

50

3

100

900

100

4

200

800

200

5

400

600

400

6

600

400

600

7

800

200

800

8

1000

0

1000

*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknown. You are free to adapt the concentrations to be tested to your necessities.

e-BQC SPECIFICITY

Ascorbic acid is more predominantly present in Q1 where it presents lower detection limits and fewer interferences. It is recommended to use Q1 for calibrations at low concentrations of ascorbic acid.

 

Q1

Q2

Qt

Sensitivity

0.07 µC/ µM

0.04 µC/ µM

0.10 µC/ µM

LOD

2 µM

10 µM

12 µM

LOQ

3 µM

16 µM

19 µM

LOB

1.7 µM

9.2 µM

10.9 µM

RSD

8 %

5 %

5 %

 

COMPARISON WITH TAC ASSAY KITS

If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with an ascorbic acid standard could be used:

Product

Reference

ABTS

KF01002

FRAP

KF01003

FAST FRAP

KF01006

  

 

Additional information

vol

5 ml

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