Name: KB03006 | Polyphenol Quantification Assay Kit (Folin Ciocalteu Method)
SKU: KB03006
Availability: InStock

KB03006 | Polyphenol Quantification Assay Kit (Folin Ciocalteu Method)

Price range: 225.00€ through 430.00€ TAX excl.

BQC Polyphenol Quantification Assay Kit (Folin Ciocalteu Method) is a quick, easy, and reproducible assay to quantify phenolic compounds in a wide variety of samples:

KB03006A is for samples/extracts prepared using aqueous solvents
KB03006B is for samples/extracts prepared using organic solvents

This Phenolic Quantification Assay Kit is based on the Folin Ciocalteu (FC) method. In this method, the Folin Ciocalteau Reagent (FCR) oxidizes phenolic compounds to phenolates at alkaline pH resulting in the formation of a blue colored molybdenum-tungsten complex. The reduced FCR can be spectrophotometrically detected in the range of 690 to 710 nm with a maximum of absorbance at 700 nm.

Absorbance measured at 700 nm is directly proportional to the concentration of the phenolic compounds. Generally, Gallic Acid (GA) is used as the reference standard compound for the Folin Ciocalteu assay, and the results are expressed as Gallic Acid Equivalents (GAE).

Test

Protocol

Clear selection

SKU: KB03006 Category: Phenolic Compounds Tags: Antioxidant, Folin Ciocalteu, Polyphenols

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: Room temperature

Assay time: 15 minutes

Reagents: Reagent A, Reagent B, Reagent C, and Standard (Gallic acid)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at support@bqcredox.com

To place an order choose A for general extraction procedures or B for organic solvent extraction protocol.

Protocol Booklet

Download the web version

Download the web version – organic solvents

To access the full information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

2026

Adongbede, E., Khatiwada, J. & Williams, L. (2026.) Nutraceutical Potential and Bioactivities of Wild Trametespolyzona (Pers.) Justo. Advances in Bioscience and Biotechnology, 17, 245-274. https://doi.org/10.4236/abb.2026.176017
Al Srouji, S., El Kayal, W., Ismail, D., Nasreddine, L., Gali-Muhtasib, H., & Hwalla, N. (2026). Unveiling the antioxidant and anticancer potential of local mediterranean olive and fig extracts against breast cancer. BMC Complement Med Ther,26(116). https://doi.org/10.1186/s12906-026-05296-4
Bucekova, M., Godocikova, J., Cigankova, S., Chirkova, V., Safranek, M., & Majtan, J. (2026). Divergent polyphenolic modulation of glucose oxidase activity across honey types: Implications for medical-grade honey standardization. Current research in food science, 12, 101366. https://doi.org/10.1016/j.crfs.2026.101366
Chirkova, V., Godocikova, J., Safranek, M., & Majtan, J. (2026). Physicochemical characterisation, glucose oxidase activity, and antibacterial properties of Armenian honey: quality assessment beyond conventional parameters. International Journal of Food Science and Technology, 61(1), vvag060. https://doi.org/10.1093/ijfood/vvag060
Darolt, J.C., Raiol-Junior, L.L., Carvalho, E.V., Alves, M.N., Wulff, N.A., Bagéa, J., Gros, O., Hufnagel, B., Morillon, R., Peña, L. & Girardi, E.A. (2026). Can an HLB-resistant interstock block the long-distance movement of ‘Candidatus Liberibacter asiaticus’ within citrus trees?. Frontiers in Plant Science, 17, 1733981. doi: 10.3389/fpls.2026.1733981
Nuzzo, D., Zupo, V., Esposito, R., Somma, E., Savarese, C., Carfagna, S., Salbitani, G., Panunzi, S., Pompa, M., De Gaetano, A., Girgenti, A., Palumbo, L., Picone, P., & Costantini, M. (2026). Effects of lighting technologies on the physiology of a marine diatom, Cocconeis scutellum parva (Bacillariophyceae): 2. Production of antioxidants and other bioactive compounds. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 302, 110451. https://doi.org/10.1016/j.cbpc.2026.110451
Yu, J., Jung, M., Kim, J., & Bai, J. (2026). Improving sensory quality and antioxidant functionality of brewer’s spent yeast via combined heat and slightly acidic electrolyzed water treatment. Food Bioscience, 79, 108651. https://doi.org/10.1016/j.fbio.2026.108651

2025

Aindelis, G., Spyridopoulou, K., Kyriakou, S., Tiptiri-Kourpeti, A., Panayiotidis, M. I., Pappa, A., & Chlichlia, K. (2025). Evaluating the chemical composition and antitumor activity of Origanum vulgare hirtum essential oil in a preclinical colon cancer model. International Journal of Molecular Sciences, 26(10), 4737. https://doi.org/10.3390/ijms26104737
Griffin, S. A. (2025). Thesis (No. 145). Louisiana Tech University. https://digitalcommons.latech.edu/theses/145
Wang, Z., Svyantek, A., & Miller, Z. (2025). Effect of blue and red light-emitting diodes on haskap (Lonicera caerulea) postharvest storage. Agricultural Production, Processing and Storage, 1(3). https://doi.org/10.1007/s44462-025-00006-8
Wang, Z., Svyantek, A., Miller, Z., Davis, H., & Kapus, A. (2025). The influence of ultraviolet-C light pretreatment on blackcurrant (Ribes nigrum) quality during storage. Applied Sciences, 15(15), 8452. https://doi.org/10.3390/app15158452

2024

Pudelka, L., Sleha, R., Janovska, S., Radochova, V., & Bostik, P. (2024). Czech honeydew honeys—A potential source of local medical honey with strong antimicrobial activity. Pharmaceuticals, 17(7), 840. https://doi.org/10.3390/ph17070840
Tragkola, V., Anestopoulos, I., Kyriakou, S., Amery, T., Franco, R., Pappa, A., & Panayiotidis, M. I. (2024). Naturally-derived phenethyl isothiocyanate modulates apoptotic induction through regulation of the intrinsic cascade and resulting apoptosome formation in human malignant melanoma cells. Toxicology Mechanisms and Methods, 34(9), 985–999. https://doi.org/10.1080/15376516.2024.2369666
Wang, Z., Svyantek, A., Miller, Z., & Watrelot, A. A. (2024). Fermentation process effects on fermented McIntosh apple ciders. Fermentation, 10(3), 115. https://doi.org/10.3390/fermentation10030115

2023

Bonnin, M., Favreau, B., Soriano, A., Leonhardt, N., Oustric, J., Lourkisti, R., Ollitrault, P., Morillon, R., Berti, L., & Santini, J. (2023). Insight into physiological and biochemical determinants of salt stress tolerance in tetraploid citrus. Antioxidants, 12(8), 1640. https://doi.org/10.3390/antiox12081640
D’Agostino, C., Chillocci, C., Polli, F., Surace, L., Simonetti, F., Agostini, M., Brutti, S., Mazzei, F., Favero, G., & Zumpano, R. (2023). Smartphone-based electrochemical biosensor for on-site nutritional quality assessment of coffee blends. Molecules, 28(14), 5425. https://doi.org/10.3390/molecules28145425
Darwiche, L., Mesmar, J., Baydoun, E., & El Kayal, W. (2023). In Vitro Evaluation of Biological and Anticancer Activities Exhibited by Five Varieties of Vitis viniferaInternational Journal of Molecular Sciences, 24(17), 13440. https://doi.org/10.3390/ijms241713440
Del Mar Rivas-Chacón, L., Yanes-Díaz, J., De Lucas, B., Riestra-Ayora, J. I., Madrid-García, R., Sanz-Fernández, R., & Sánchez-Rodríguez, C. (2023). Cocoa polyphenol extract inhibits cellular senescence via modulation of SIRT1 and SIRT3 in auditory cells. Nutrients, 15(3), 544. https://doi.org/10.3390/nu15030544
Hamie, N., Nacouzi, D., Choker, M., Salameh, M., Darwiche, L., & Kayal, W. E. (2023). Maturity assessment of different table grape cultivars grown at six different altitudes in Lebanon. Plants, 12(18), 3237. https://doi.org/10.3390/plants12183237
Heredia, R. F., Riestra-Ayora, J. I., Yanes-Díaz, J., Vasallo, I. J. T., Andreu-Vázquez, C., Sanz-Fernández, R., & Sánchez-Rodríguez, C. (2023). Cocoa polyphenols prevent age-related hearing loss and frailty in an in vivo Antioxidants, 12(11), 1994. https://doi.org/10.3390/antiox12111994
Kyriakou, S., Tragkola, V., Paraskevaidis, I., Plioukas, M., Trafalis, D. T., Franco, R., Pappa, A., & Panayiotidis, M. I. (2023). Chemical characterization and biological evaluation of Epilobium parviflorum extracts in an in vitro model of human malignant melanoma. Plants, 12(8), 1590. https://doi.org/10.3390/plants12081590
Nacouzi, D., Masry, R., & Kayal, W. E. (2023). Quality and phytochemical composition of sweet cherry cultivars can be influenced by altitude. Plants, 12(12), 2254. https://doi.org/10.3390/plants12122254
Ververis, A., Ioannou, K., Kyriakou, S., Violaki, N., Panayiotidis, M. I., Plioukas, M., & Christodoulou, K. (2023). Sideritis scardica extracts demonstrate neuroprotective activity against Aβ25–35 toxicity. Plants, 12(8), 1716. https://doi.org/10.3390/plants12081716

2022

Chin, H. Y. (2022). Sensory evaluation, physicochemical properties and bioactivities of Apis cerana honey with ginger (Final Year Project, UTAR). http://eprints.utar.edu.my/4901/
Del Mar Rivas-Chacón, L., Yanes-Díaz, J., De Lucas, B., Riestra-Ayora, J. I., Madrid-García, R., Sanz-Fernández, R., & Sánchez-Rodríguez, C. (2022). Preventive effect of cocoa flavonoids via suppression of oxidative stress-induced apoptosis in auditory senescent cells. Antioxidants, 11(8), 1450. https://doi.org/10.3390/antiox11081450
Kyriakou, S., Tragkola, V., Alghol, H., Anestopoulos, I., Amery, T., Stewart, K., Winyard, P. G., Trafalis, D. T., Franco, R., Pappa, A., & Panayiotidis, M. I. (2022). Evaluation of bioactive properties of lipophilic fractions of edible and non-edible parts of Nasturtium officinale (watercress) in a model of human malignant melanoma cells. Pharmaceuticals, 15(2), 141. https://doi.org/10.3390/ph15020141
Pełka, K., Bucekova, M., Godocikova, J., Szweda, P., & Majtan, J. (2022). Glucose oxidase as an important yet overlooked factor determining the antibacterial activity of bee pollen and bee bread. European Food Research and Technology, 248(12), 2929–2939. https://doi.org/10.1007/s00217-022-04101-z
Yip, S. C. (2022). Influence of addition of ginger on sensory quality, physicochemical properties and bioactivities of Malaysian multifloral honey (Final Year Project, UTAR). http://eprints.utar.edu.my/4909/

2021

Kyriakou, S., Tragkola, V., Plioukas, M., Anestopoulos, I., Chatzopoulou, P. S., Sarrou, E., Trafalis, D. T., Deligiorgi, M. V., Franco, R., Pappa, A., & Panayiotidis, M. I. (2021). Chemical and biological characterization of the anticancer potency of Salvia fruticosa in a model of human malignant melanoma. Plants, 10(11), 2472. https://doi.org/10.3390/plants10112472

2020

Chen, Y., Huang, S., Tu, J., Yu, J., Nurlatifah, A. O., Chiu, W., Su, Y., Chang, H., Putri, D. A., & Cheng, H. (2020). Exopolysaccharides of Bacillus amyloliquefaciens modulate glycemic level in mice and promote glucose uptake of cells through the activation of Akt. International Journal of Biological Macromolecules, 146, 202–211. https://doi.org/10.1016/j.ijbiomac.2019.12.217
Lam, E., Li, R., Rodrigues, V., Vires, L., Adams, R., Sherrill, J., & Oblong, J. (2020). Enhanced retinoid response by a combination of the vitamin A ester retinyl propionate with niacinamide and a flavonoid containing Ceratonia siliqua extract in retinoid responsive in vitro International Journal of Cosmetic Science, 43(1), 102–106. https://doi.org/10.1111/ics.12669

2019

Bucekova, M., Jardekova, L., Juricova, V., Bugarova, V., Di Marco, G., Gismondi, A., Leonardi, D., Farkasovska, J., Godocikova, J., Laho, M., Klaudiny, J., Majtan, V., Canini, A., & Majtan, J. (2019). Antibacterial activity of different blossom honeys: New findings. Molecules, 24(8), 1573. https://doi.org/10.3390/molecules24081573
Boboc, M. G., & Dumitrescu, E. (2019). Testing the pharmaco-therapeutic efficacy of the alcoholic extract of Centella asiatica on the cell line HaCat. Lucrări Ştiinţifice Medicina Veterinară, LII(1). http://www.usab-tm.ro/USAMVBT_Revista_ro_1086.html

2018

Bucekova, M., Buriova, M., Pekarik, L., Majtan, V., & Majtan, J. (2018). Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-27449-3
Bucekova, M., Juricova, V., Di Marco, G., Gismondi, A., Leonardi, D., Canini, A., & Majtan, J. (2018). Effect of thermal liquefying of crystallised honeys on their antibacterial activities. Food Chemistry, 269, 335–341. https://doi.org/10.1016/j.foodchem.2018.07.012

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KB03006 | Polyphenol Quantification Assay Kit (Folin Ciocalteu Method)