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KF01004 | ORAC Assay Kit | Antioxidant Capacity

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KF01004 | ORAC Assay Kit | Antioxidant Capacity

220.00528.00 TAX excl.

The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein. As a result, there is a change in fluorescent intensity. Hence, the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.

It is possible to quantify the protection by calculating the area under the curve (AUC) from the experimental sample. After subtracting the AUC for the blank, the resultant difference would be the protection that the antioxidant compound confers. Trolox®, a water-soluble vitamin E analog, is used as the calibration standard and ORAC results are expressed as Trolox® equivalents.

Check our blog post about ORAC Data Analysis 

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: -20ºC,and 4ºC

Assay time: 100 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

  • Narciso JO, and Nyström L. 2020. “Breathing New Life to Ancient Crops: Promoting the Ancient Philippine Grain ‘Kabog Millet’ as an Alternative to Rice.” Foods 9 (12): 1727. https://doi.org/10.3390/foods9121727
  • Narciso JO, and Nyström L. n.d. “Proceedings 2020, 4, x;.” Accessed December 9, 2020. Presented at the 1st International Electronic Conference on Food Science and Functional Foods, 10–25 November 2020; Available online: https://foods_2020.sciforum.net
  • Narciso JO, Nyström L. 2020. “Alternative Grain Crops: Introducing the Kabog Millet from the Philippines as a Functional Food Ingredient”. doi:10.3390/foods_2020-07740

FAQs

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