BQC ORAC (Oxygen Radical Antioxidant Capacity) Assay Kit is a powerful tool to measure total antioxidant capacity (TAC) of biological samples, cell lysates, tissue homogenates, food, and beverages.
The ORAC Assay is based on the inhibition of fluorescein oxidation by peroxyl radical in the presence of antioxidants. Peroxyl radical (ROO·) is produced by a free radical initiator (AAPH: 2,2’-azobis-2- methyl propanimidamide dihydrochloride) which produces a fluorescent quenching of fluorescein over time. The presence of antioxidants inhibits the free radical damage to fluorescein, resulting in a preservation of the fluorescent signal.
The sample antioxidant capacity correlates to the area under the fluorescence decay curve (AUC). The AUC is used to determine the antioxidant activity in a sample and is compared to an antioxidant standard curve prepared using the water-soluble vitamin E analog Trolox as standard. ORAC results are expressed as Trolox equivalents (TEAC).
Check our blog post about ORAC Data Analysis
Select your protocol when ordering:
- KF01004A. Analysis of hydrophilic antioxidants (aqueous samples/extracts)
- KF01004B. Analysis is for lipophilic antioxidants (organic extracts)
