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KF01004 | ORAC Assay Kit | Antioxidant Capacity

220.00528.00 TAX excl.

The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein. As a result, there is a change in fluorescent intensity. Hence, the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.

It is possible to quantify the protection by calculating the area under the curve (AUC) from the experimental sample. After subtracting the AUC for the blank, the resultant difference would be the protection that the antioxidant compound confers. Trolox®, a water-soluble vitamin E analog, is used as the calibration standard and ORAC results are expressed as Trolox® equivalents.

Check our blog post about ORAC Data Analysis 


Additional information


100, 200, 400


  • Narciso, Joan Oñate, and Laura Nyström. 2020. “Breathing New Life to Ancient Crops: Promoting the Ancient Philippine Grain ‘Kabog Millet’ as an Alternative to Rice.” Foods 9 (12): 1727. https://doi.org/10.3390/foods9121727
  • Narciso, Joan Oñate, and Laura M Nyström. n.d. “Proceedings 2020, 4, x;.” Accessed December 9, 2020. Presented at the 1st International Electronic Conference on Food Science and Functional Foods, 10–25 November 2020; Available online: https://foods_2020.sciforum.net
  • Joan Oñate Narciso and Laura M. Nyström (2020) Alternative Grain Crops: Introducing the Kabog Millet from the Philippines as a Functional Food Ingredient †


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