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KB03010 | Nitrite/Nitrate Determination Assay Kit

KB03010 | Nitrite/Nitrate Determination Assay Kit

320.00760.00 TAX excl.

Nitrate/Nitrite Determination Assay Kit is recommended for the determination of nitrite and nitrate.

BQC Nitrite/Nitrate Assay Kit is based on the Griess method. Nitrite reacts with Griess reagent to produce an azo compound that can be spectrophotometrically quantified at 540 nm. Nitrate is converted to nitrite by the enzyme nitrate reductase.

Total nitrite (NO2-) can be therefore determined following the two step protocol: enzymatic reduction + Griess reaction. Nitrite (NO2-) concentration naturally present in the sample can be directly quantify using the Griees reaction. Nitrate (NO3-) concentration can be calculated by subtracting nitrite concentration naturally present in the sample from the total nitrite concentration.

 

Subject to availability.

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: -20ºC, and 4ºC

Assay time: 150 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, Reagent F and Standard (NaNO2)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

Download the web version

 

 

 

 

 

 

For more information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

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FAQs

Check the Frequently Asked Questions section here.

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