KB03010 | Nitrite/Nitrate Determination Assay Kit

Nitrate/Nitrite Determination Kit is recommended for the determination of nitrite and nitrate, which is a method for the detection of nitric oxide formation. The assay described here measures the nitrite and nitrate anions. Firstly, the nitrate is reduced to nitrite, catalyzed by the nitrate reductase, with cofactors and specific compounds to eliminate interferences.

The detection is based on the final product detection (diazonium compound, λmax= 540 nm) obtained after the nitrite reaction in several steps with sulfanilamide.