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KB03010 | Nitrite/Nitrate Determination Assay Kit

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KB03010 | Nitrite/Nitrate Determination Assay Kit

305.00415.00 TAX excl.

Nitrate/Nitrite Determination Kit is recommended for the determination of nitrite and nitrate, which is a method for the detection of nitric oxide formation. The assay described here measures the nitrite and nitrate anions. Firstly, the nitrate is reduced to nitrite, catalyzed by the nitrate reductase, with cofactors and specific compounds to eliminate interferences.

The detection is based on the final product detection (diazonium compound, λmax= 540 nm) obtained after the nitrite reaction in several steps with sulfanilamide.

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: -20ºC, and 4ºC

Assay time: 150 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, Reagent F and Standard (NaNO2)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

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Certificate of Analysis (CoA)

CoA_Nitrite_Nitrate_30100807

Safety Data Sheet (SDS)

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