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KF03001B | MTT-B Cell Proliferation Assay

160.00430.00 TAX excl.

MTT-B Cell Proliferation Assay is based on the transformation and colorimetric quantification of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The respiratory chain and other electron transport systems reduce MTT and other tetrazolium salts. As a result, non-water-soluble violet formazan crystals accumulate within the cell. The amount of these crystals can be determined using a spectrophotometer, serving as an estimate of the number of mitochondria and, hence, the number of living cells in the sample. These features allow performing cytotoxicity or cell proliferation assays.

In this assay, the resulting formazan crystals are directly redissolved without removing the culture medium. This kit is suitable for cells in suspension and faster, but less sensitive, than MTT Cell Proliferation Assay A.

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Additional information

Test

500, 2000, 5000

Publications

  • Cid-Barrio, Laura, Diego Bouzas-Ramos, Alfonso Salinas-Castillo, Yasumitsu Ogra, Jorge Ruiz Encinar, and José M. Costa-Fernández. 2020. “Quantitative Assessment of Cellular Uptake and Differential Toxic Effects of HgSe Nanoparticles in Human Cells.” Journal of Analytical Atomic Spectrometry 35 (9): 1979–88. https://doi.org/10.1039/d0ja00162g.
  • Escudero-Francos, María A., Vanesa Cepas, Pedro González-Menéndez, Rosana Badía-Laíño, Marta E. Díaz-García, Rosa M. Sainz, Juan C. Mayo, and David Hevia. 2017. “Cellular Uptake and Tissue Biodistribution of Functionalized Gold Nanoparticles and Nanoclusters.” Journal of Biomedical Nanotechnology 13 (2): 167–79. https://doi.org/10.1166/jbn.2017.2344
  • Mayo, Juan C., David Hevia, Isabel Quiros-Gonzalez, Aida Rodriguez-Garcia, Pedro Gonzalez-Menendez, Vanesa Cepas, Iván Gonzalez-Pola, and Rosa M. Sainz. 2017. “IGFBP3 and MAPK/ERK Signaling Mediates Melatonin-Induced Antitumor Activity in Prostate Cancer.” Journal of Pineal Research 62 (1). https://doi.org/10.1111/jpi.12373
  • Rodriguez-Garcia, Aida, David Hevia, Juan C. Mayo, Pedro Gonzalez-Menendez, Lucia Coppo, Jun Lu, Arne Holmgren, and Rosa M. Sainz. 2017. “Thioredoxin 1 Modulates Apoptosis Induced by Bioactive Compounds in Prostate Cancer Cells.” Redox Biology 12 (December 2016): 634–47. https://doi.org/10.1016/j.redox.2017.03.025
  • Escudero-Francos, María A., Vanesa Cepas, Pedro González-Menéndez, Rosana Badía-Laíño, Marta E. Díaz-García, Rosa M. Sainz, Juan C. Mayo, and David Hevia. 2017. “Cellular Uptake and Tissue Biodistribution of Functionalized Gold Nanoparticles and Nanoclusters.” Journal of Biomedical Nanotechnology 13 (2): 167–79. https://doi.org/10.1166/jbn.2017.2344.
  • Cid-Barrio, L., Bouzas-Ramos, D., Salinas-Castillo, A., Ogra, Y., Encinar, J. R., & Costa-Fernandez, J. M. (2020). Quantitative assessment of cellular uptake and differential toxic effects of HgSe nanoparticles in human cells. Journal of Analytical Atomic Spectrometry.

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