KF03001A | MTT Cell Proliferation Assay Kit (adherents cells)
170.00€ – 455.00€ TAX excl.
MTT-A Cell Proliferation Assay is based on the transformation and colorimetric quantification of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The respiratory chain and other electron transport systems reduce MTT and other tetrazolium salts. As a result, non-water-soluble violet formazan crystals accumulate within the cell. The amount of these crystals can be determined using a spectrophotometer, serving as an estimate of the number of mitochondria and, hence, the number of living cells in the sample. These features allow performing cytotoxicity or cell proliferation assays, which are widely used in immunology, toxicology, and cellular biology.
In the MTT-A cell proliferation assay is necessary to remove the cell medium before resuspending MTT crystals formed.
Additional information
Sizes: 500,2000 and 5000 tests
Expiry date: 1 year
Storage: -20ºC, and room temperature
Assay time: 5 hours
Reagents: MTT solution and MTT solvent
Necessary material: 96 well-plate spectrophotometer
If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com
Protocol Booklet
Download the web version
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Safety Data Sheet (SDS)
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Publications
- Rodriguez A, Hevia D, Mayo JC, et al. 2017. “Thioredoxin 1 modulates apoptosis induced by bioactive compounds in prostate cancer cells.” Redox Biology 12 (December 2016): 634–47. https://doi.org/10.1016/j.redox.2017.03.025
- Mayo JC, Hevia D, Quiros I, et al. 2017. “IGFBP3 and MAPK/ERK signaling mediates atonin-induced antitumor activity in prostate cancer.” Journal of Pineal Research 62 (1). https://doi.org/10.1111/jpi.12373
- Escudero MA, Cepas V, González P, et al. 2017. “Cellular uptake and tissue biodistribution of functionalized gold nanoparticles and nanoclusters.” Journal of Biomedical Nanotechnology 13 (2): 167–79. https://doi.org/10.1166/jbn.2017.2344.
- Cid L, Bouzas D, Salinas A, et al. 2020. “Quantitative assessment of cellular uptake and differential toxic effects of HgSe nanoparticles in human cells”. J. Anal. At. Spectrom. 35, 1979-1988. https://doi.org/10.1039/D0JA00162G.
FAQs
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