KF03001 | MTT Cell Proliferation Assay Kit

178.00477.00 TAX excl.

MTT Cell Proliferation Assay is based on the transformation and colorimetric quantification of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The respiratory chain and other electron transport systems reduce MTT and other tetrazolium salts. As a result, non-water-soluble violet formazan crystals accumulate within the cell. The amount of these crystals can be determined using a spectrophotometer, serving as an estimate of the number of mitochondria and, hence, the number of living cells in the sample. These features allow performing cytotoxicity or cell proliferation assays.

The principle of the MTT Cell Proliferation Assay is based in mitochondrial activity. For viable cells, mitochondrial activity is constant and thereby an increase or decrease in the number of viable
cells is linearly related to mitochondrial activity.

You can follow two protocols for this assay:

  • In MTT – A protocol is necessary to remove the cell medium before resuspending MTT crystals formed.
  • Using MTT – B protocol, the resulting formazan crystals are directly redissolved without removing the culture medium. This kit is suitable for cells in suspension and faster, but less sensitive, than MTT Cell Proliferation Assay A.
SKU: KF03001-1Categories: Tags: ,

Additional information

Sizes: 500,2000 and 5000 tests

Expiry date: 1 year

Storage: -20ºC, and room temperature

Assay time: 300 minutes

Reagents: MTT solution and MTT solvent

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

Download the web version

 

 

 

 

 

 

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Safety Data Sheet (SDS)

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Publications

  • Rodriguez A, Hevia D, Mayo JC, et al. 2017. “Thioredoxin 1 modulates apoptosis induced by bioactive compounds in prostate cancer cells.” Redox Biology 12 (December 2016): 634–47. https://doi.org/10.1016/j.redox.2017.03.025
  • Mayo JC, Hevia D, Quiros I, et al. 2017. “IGFBP3 and MAPK/ERK signaling mediates atonin-induced antitumor activity in prostate cancer.” Journal of Pineal Research 62 (1). https://doi.org/10.1111/jpi.12373
  • Escudero MA, Cepas V, González P, et al. 2017. “Cellular uptake and tissue biodistribution of functionalized gold nanoparticles and nanoclusters.” Journal of Biomedical Nanotechnology 13 (2): 167–79. https://doi.org/10.1166/jbn.2017.2344.
  • Cid L, Bouzas D, Salinas A, et al. 2020. “Quantitative assessment of cellular uptake and differential toxic effects of HgSe nanoparticles in human cells”. J. Anal. At. Spectrom. 35, 1979-1988. https://doi.org/10.1039/D0JA00162G.

FAQs

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