KB03016 | MDA-TBARs Assay Kit | MDA Quantification

210.00503.00 TAX excl.

Among reactive aldehydes, malondialdehyde (MDA) has been extensively used as an index of LPO.

Malondialdehyde (MDA) can be generated by oxidizing agents that alter lipid structure, creating lipid peroxides. The TBARS Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. This method, is convenient to determine the relative lipid peroxide content of samples, including plasma, serum, cell culture supernatants, and urine samples. Multi-unsaturated lipids are most likely to form peroxides, and so they are the most reactive lipids in the TBARs assay.

 

NOTE: Flat bottom transparent microplates for UV/VIS applications is sent by default. Pelase, ask for black microplates for fluorescence measurements.

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC

Assay time: 120 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, Reagent E, and Standard (MDA)

Necessary material: 96 well-plate spectrophotometer. NOTE: Flat bottom transparent microplates for UV/VIS applications is sent by default. Pelase, ask for black microplates for fluorescence measurements.

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

Download the web version

 

 

 

 

 

 

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Safety Data Sheet (SDS)

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Publications

  • Garcia J., Turiel D, Bettmer J, et al. 2020. “In vitro and in situ experiments to evaluate the biodistribution and cellular toxicity of ultrasmall iron oxide nanoparticles potentially used as oral iron supplements”. Nanotoxicology,14(3):388-403. doi: 10.1080/17435390.2019.1710613
  • Chaklader MR, Siddik MAB, Fotedar R. 2020. “Total replacement of fishmeal with poultry by-product meal affected the growth, muscle quality, histological structure, antioxidant capacity and immune response of juvenile barramundi, Lates calcarifer”. 2020 Nov 12;15(11):e0242079. doi:10.1371/journal.pone.0242079
  • Buyse K, Delezie E, Goethals L, et al. 2021.”Chestnut tannins in broiler diets: performance, nutrient digestibility, and meat quality.” Poultry Science. 10,12. https://doi.org/10.1016/j.psj.2021.101479
  • Ahles S, Cuijpers I, Hartgens F, et al. 2022. “The Effect of a Citrus and Pomegranate Complex on Physical Fitness and Mental Well-Being in Healthy Elderly: A Randomized Placebo-Controlled Trial”. J Nutr Health Aging . DOI: 10.1007/s12603-022-1834-4
  • Crescitelli MC, Simon I, Ferrini L, Calvo H, Torres AM, Cabero I, Panedas MM, Rauschemberger MB, Aguirre MV, Rodríguez JP, Hernández M, Nieto ML. Anti-Neuroinflammatory Potential of a Nectandra angustifolia (Laurel Amarillo) Ethanolic Extract. Antioxidants. 2023; 12(2):232. https://doi.org/10.3390/antiox12020232
  • Rebollo-Hernanz M, Bringe NA, Gonzalez de Mejia E. Selected Soybean Varieties Regulate Hepatic LDL-Cholesterol Homeostasis Depending on Their Glycinin:β-Conglycinin Ratio. Antioxidants. 2023; 12(1):20. https://doi.org/10.3390/antiox12010020
  • Pinazo-Durán, Maria D., et al. “Biochemical–molecular–genetic biomarkers in the tear film, aqueous humor, and blood of primary open-angle glaucoma patients.” Frontiers in Medicine 10 (2023): 1157773

FAQs

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