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info@bioquochem.com(+34) 985 26 92 92

KB03002 | LPO Assay Kit | Lipid Peroxidation (MDA + HNE)

189.00454.00 TAX excl.

LPO assay kit measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, an acid-catalyzed attack at the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a result, this reaction gives a diindolylalkane (chromophore) with maximum absorbance in the region of 580-620 nm.

Malondialdehyde (MDA) and 4-Hydroxynonenal (4-HNE) are important toxic byproducts of lipid peroxidation. So, the measurement of the amounts of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-HNE is a major product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, increased levels of HNE were found in plasma and various organs under oxidative stress conditions. In fact, MDA is in many instances the most abundant individual aldehyde resulting from lipid peroxidation. In vitro, MDA can alter proteins, DNA, RNA, and many other biomolecules.

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Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC and room temperature

Assay time: 60 minutes

Reagents: LPO solvent, Reagent A, Reagent B, and Standard (1,1,3,3-tetrametoxipropano)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Certificate of Analysis (CoA)

CoA_LPO_30020412

CoA_LPO_30020605

CoA_LPO_30020906

CoA_LPO_30021902

CoA_LPO_30022605

CoA_LPO_30022611

CoA_LPO_30022904

CoA_LPO_30022909

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

  • Reyes C, Ramiro D, Rodríguez P, et al. 2018. “Effects of Arachidonic and Docosohexahenoic Acid Supplementation during Gestation in Rats. Implication of Placental Oxidative Stress.” International Journal of Molecular Sciences 19 (12). https://doi.org/10.3390/ijms19123863.
  • Flores J, Padilla E , Fernandez O, Diaz N, et al. 2019. “Gastroprotective Activity and Pharmacological Safety Evaluation of Eupatorium Aschenbornianum.” Experimental and Therapeutic Medicine 18 (October): 4467–72. https://doi.org/10.3892/etm.2019.8093.
  • Garcia J, Turiel D, Bettmer J, et al. 2020. “In Vitro and in Situ Experiments to Evaluate the distribution and Cellular Toxicity of Ultrasmall Iron Oxide Nanoparticles Potentially Used as Oral Iron Supplements.” Nanotoxicology 14 (3): 388–403. https://doi.org/10.1080/17435390.2019.1710613.
  • Gila A, Herranz G, Cañas S, et al. 2020. “Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation”. Nutrients, 12(9), 2569. https://doi.org/10.3390/nu12092569.
  • Phuthong S, Reyes CG, Rodríguez P, et al. 2020. “Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition” Int J Mol Sci. 2020 Dec 28;22(1):237. DOI: 10.3390/ijms22010237
  • Ahmed MA, Kamel EO. 2020. “Involvement of H 2 S, NO and BDNF‐TrkB signalling pathway in the protective effects of simvastatin against pentylenetetrazole‐induced kindling and cognitive impairments”. Basic & Clinical Pharmacology & Toxicology. 127:461-476 https://doi.org/10.1111/bcpt.13457
  • Martín M, Aller R, Heredia M, et al. 2021. “Lipid peroxidation as a hallmark of severity in COVID-19 patients.” Redox Biology. 48: 102181. https://doi.org/10.1016/j.redox.2021.102181
  • Lulijwa R, Young T, Symonds J, Walker S, et al. 2021. “Uncoupling Thermotolerance and Growth Performance in Chinook Salmon: Blood Biochemistry and Immune Capacity.” Metabolites, 11, 547. https://doi.org/10.3390/metabo11080547
  • Montes P , Guerra A, García P, et al. 2022. “Effect of 5-Azacitidine Treatment on Redox Status and Inflammatory Condition in MDS Patients.” Antioxidants, 11, 139. https://doi.org/10.3390/antiox11010139

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