BQC HORAC (Hydroxyl Radical Antioxidant Capacity) Assay Kit is a powerful tool to measure the antioxidant capacity of biological samples, cell lysates, tissue homogenates, food, and beverages.
The HORAC Assay is based on the inhibition of fluorescein oxidation by hidroxyl radical in the presence of antioxidants. Hidroxyl radicals are generated from H2O2 by a Fenton like reaction. These radicals quench fluorescein fluorescence over time. The presence of antioxidants inhibits the free radical damage to fluorescein, resulting in a preservation of the fluorescent signal.
The sample antioxidant capacity correlates to the area under the fluorescence decay curve (AUC). The AUC is used to determine the antioxidant activity in a sample and is compared to an antioxidant standard curve prepared using Gallic Acid as standard. HORAC results are expressed as Galic Acid concentration (mM GA).
