BQC Peroxide Assay Kit is a quick, easy, and reproducible assay for peroxides quantification in several biological samples.
Reactive oxygen (ROS) and nitrogen (RNS) species are produced in metabolic and physiological processes and can be overproduced in response to different stimuli. Organisms have different enzymatic and non-enzymatic antioxidative mechanisms to ameliorate the harmful effects of these oxidant species. An imbalance between oxidant species and the antioxidant defense system leads to an oxidative stress state condition that causes damage to cellular biomolecules, and it is involved in the development of several diseases (e.g. cancer, Parkinson, Alzheimer). Oxidative stress can be evaluated directly by measuring ROS. Major ROS include superoxide, hydroxyl radicals and peroxides.
BQC Peroxide Assay Kit is based on the chromogenic Fe3+-xylenol orange reaction. Peroxides present in the sample oxidizes ferrous ion (Fe2+) to ferric ion (Fe3+). In an acidic medium the generated Fe3+ complexes with xylenol orange dye to yield a colored product with maximum absorbance at 580 nm. The amount of complex generated is proportional to the total peroxide concentration in the sample. In this kit H2O2 is used as standard and results are expressed as H2O2 equivalents (µM H2O2).
