Ascorbate Peroxidase (APX) is a key enzyme in the ascorbate-glutathione (AsA-GSH) cycle, one of the most vital systems in plant antioxidant defense. APX catalyzes the reduction of hydrogen peroxide  into water, using ascorbate as a specific electron donor, and generating monodehydroascorbate (MDHA) as a byproduct.

Monitoring APX activity provides valuable insight into a plant’s redox status and overall oxidative stress response.

The BQC APX Activity Assay Kit offers a simple, rapid, and reproducible method to quantify APX activity in plant samples. APX catalyzes the conversion of H2O2 to H2O using ascorbate as the specific electron donor. In this APX assay kit, APX activity is determined by the rate of ascorbate oxidation at 290 nm.