Edificio CEEI, Parque Tecnológico de Asturias, 33428, Asturias   | info@bioquochem.com

(+34) 985 26 92 92  | linkedin.com/bioquochem

info@bioquochem.com(+34) 985 26 92 92

KB03033 | NAD/NADH quantification assay kit

405.001,100.00 TAX excl.

NAD/NADH quantification assay kit is based on several reactions involving various chemical compounds. The concentration of NAD+ is calculated through its activity as a cofactor of the reaction of the enzyme alcohol dehydrogenase (ADH) with ethanol. In a situation of maximum enzyme-substrate saturation, the rate depends solely on the concentration of available NAD+, which is transformed in its reduced form NADH.

The measurement of absorbance is carried out thanks to the ability of NADH to cede an electron to an intermediate electron donor: 1-methoxyPMS. This intermediate donor will cede the electron to MTT which will turn in formazan (from yellow to blue), absorbing at a wavelength of 570 nm.

Clear

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: -20, 4ºC and room temperature

Assay time: 90 minutes

Reagents: Solvent, Reagent A, Reagent B, Reagent C, Reagent D, and Standard (NAD)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

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Safety Data Sheet (SDS)

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