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KF01003 | FRAP Assay Kit | Antioxidant Capacity

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KF01003 | FRAP Assay Kit | Antioxidant Capacity

190.00430.00 TAX excl.

This assay measures the ferric reducing ability of plasma (FRAP). At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), intense blue color with an absorption maximum at 593 nm develops. This reaction is nonspecific and any half-reaction which has a less-positive redox potential, under reaction conditions, then the Fe3+/Fe2+ complex half-reaction will drive Fe3+ complex reduction. Acidic conditions favor the reduction of the complex and, thereby, color development, showing that an antioxidant is present.

FRAP assay kit is recommended for total antioxidant activity of single antioxidants in an aqueous solution and added to the plasma.

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: Room temperature

Assay time: <30 minutes

Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Certificate of Analysis (CoA)

CoA_FRAP_10030907

CoA_FRAP_10031305

CoA_FRAP_10032212

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

  • Rydlová L, Tesařová E, Valentová K, and Křen V. 2017. “Enzymatic modifications of bioactive flavonoids diplomová.” Univerzita Karlova V Praze.
  • Valentová K, Káňová K, Di Meo F, Pelantová H, et al. 2017. “Chemoenzymatic preparation and biophysical properties of sulfated quercetin metabolites.” International Journal of Molecular Sciences 18 (11): 1–17. https://doi.org/10.3390/ijms18112231
  • Fernández J, García L, Monte J, et al. 2018. “Functional anthocyanin-rich sausages diminish colorectal cancer in an animal model and reduce pro-inflammatory bacteria in the intestinal microbiota.” Genes 9 (3). https://doi.org/10.3390/genes9030133.
  • Fernández, Javier. 2018. “Modulación de procesos inflamatorios y neoplásicos en colon mediante alimentos funcionales en modelos animales murinos.” Universidad de Oviedo.
  • Valentová K, Purchartová K, Rydlová L, et al. 2018. “Sulfated metabolites of flavonolignans and 2,3-Dehydroflavonolignans: preparation and properties.” International Journal of Molecular Sciences 19 (2349). https://doi.org/10.3390/ijms19082349
  • Martinez V, Nieves M, Lopez M, et al. 2018. “Tolerance to stress combination in tomato plants: new insights in the protective role of melatonin.” Molecules 23 (3). https://doi.org/10.3390/molecules23030535
  • Rios M. 2018. “Efeitos de Uma Sessão de Treino de CrossFit Em Biomarcadores Plasmáticos de Lesão Oxidativa Ficha de Catalogação.” FADEUP Porto University.
  • Fernández J, García V, Fernández M, et al. 2019. “A diet based on cured acorn ham with oleic acid content promotes anti-inflammatory gut microbiota shifts and prevents ulcerative colitis in an animal model.” Preprints, no. March. https://doi.org/10.20944/preprints201903.0005.v1.
  • Biedermann D, Moravcová V, Kateřina V, et al. 2019. “Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative.” Phytochemistry Letters 30 (January): 14–20. https://doi.org/10.1016/j.phytol.2019.01.006
  • Chambers C., Biedermann D, Valentová K, et al. 2019. “Preparation of retinoyl-flavonolignan hybrids and their antioxidant properties.” Antioxidants 8 (7): 236. doi: 10.3390/antiox8070236.
  • Rajha HN, Roula M, Richard G, Debs E, and Louka N. 2020. “‘Intensification of vaporization by decompression to the vacuum’ (IVDV), a novel technology applied as a pretreatment to improve polyphenols extraction from olive leaves.” Food Chemistry, no. Ivdv: 128236. https://doi.org/10.1016/j.foodchem.2020.128236
  • Kyriakou S, Tragkola V, Alghol H, et al. 2022. “Evaluation of Bioactive Properties of Lipophilic Fractions of Edible and Non-Edible Parts of Nasturtium officinale (Watercress) in a Model of Human Malignant Melanoma Cells.” Pharmaceuticals, 15, 141. https://doi.org/10.3390/ph15020141
  • Barba F.J., Rajha H.N., Debs E, et al. 2022, “Optimization of Polyphenols’ Recovery from Purple Corn Cobs Assisted by Infrared Technology and Use of Extracted Anthocyanins as a Natural Colorant in Pickled Turnip”. Molecules, 27(16), 5222. DOI: 10.3390/molecules27165222
  • Salem, Y.; Rajha, H.N., et al. 2022. “Stability and Antioxidant Activity of Hydro-Glyceric Extracts Obtained from Different Grape Seed Varieties Incorporated in Cosmetic Creams”. Antioxidants, 11, 1348. DOI: 10.3390/antiox11071348

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