BQC FAST FRAP(Ferric Reducing Antioxidant Power) Assay Kit is a ready-to-use, easy and highly reproducible assay to measure total antioxidant capacity (TAC) in a wide variety of samples with the outstanding feature of being faster than the FRAP classical assay.
The FRAP TAC Assay Kit is based on the reduction of Fe3+ to Fe2+ by antioxidants. This kit measures the antioxidant activity of compounds that are able to act as reductants. In the assay, at an acidic pH, the antioxidants present in the sample reduce a colorless Fe3+ complex to a blue/violet colored Fe2+ complex which shows a maximum of absorbance at 593 nm. Absorbance recorded at 593 is, therefore, directly proportional to the total antioxidant capacity.
The antioxidant potential of samples is determined based on an iron standard curve (iron standard provided with the kit) and results are expressed as Fe2+ equivalents (µM) or FRAP value.
