FAQ table

Possible cause and recommended solutions: Samples contain interfering substances > Dilute sample further (if possible) Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use Samples not deproteinized > Use an appropriate deproteinization protocol Cells/Tissue samples not homogenized completely > Repeat the sample homogenization Inappropriate

Possible cause and recommended solutions: Pipetting errors > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique

Possible cause and recommended solutions: Pipetting errors in preparation of standards > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique Standard stock is at incorrect concentration > Always refer to dilutions described in Assay preparation Improperly thawed

Possible cause and recommended solutions: Plate read at incorrect wavelength  > Check the wavelength used in the assay Incorrect microplate > Use the correct microplate for your application: UV/Vis: transparent Fluorescence: black wells/transparent bottom