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KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

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KB03011 | Superoxide dismutase (SOD) Activity Assay Kit

255.00435.00 TAX excl.

Superoxide dismutases (SODs) are metalloenzymes that catalyze the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide. SOD Activity Assay Kit bases on the reduction of WST-1, which produces a water-soluble coloured formazan dye. The rate of the reduction with a superoxide anion linearly relates to the xanthine oxidase (XO) activity. By contrast, SOD inhibits this reaction. Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Specifically, one unit of SOD corresponds to the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.

The conversion of xanthine and O2 to uric acid and H2O2 by XOD generates superoxide radicals. As a result, the superoxide anions reduce a tetrazolium salt (WST-1) to a colored formazan product (WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.

SKU: KB03011Categories: Tags: , ,

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: 4ºC

Assay time: 40 minutes

Reagents: Reagent A, Reagent B, Reagent C, and Reagent D

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com

Protocol Booklet

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To access the full information, please complete the fields. As soon as possible, the documentation will be sent to your email.


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  • Ramiro-Cortijo D, Gila-Diaz A, Herranz Carrillo G. 2022. “Influence of Neonatal Sex on Breast Milk Protein and Antioxidant Content in Spanish Women in the First Month of Lactation”. Antioxidants, 11(8), 1472. DOI: 10.3390/antiox11081472


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