Possible cause and recommended solutions:

  • Samples contain interfering substances > Dilute sample further (if possible)
  • Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use
  • Samples not deproteinized > Use an appropriate deproteinization protocol
  • Cells/Tissue samples not homogenized completely > Repeat the sample homogenization
  • Inappropriate sample dilution buffer > Refer to Assay preparation