Possible cause and recommended solutions:
- Samples contain interfering substances > Dilute sample further (if possible)
- Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use
- Samples not deproteinized > Use an appropriate deproteinization protocol
- Cells/Tissue samples not homogenized completely > Repeat the sample homogenization
- Inappropriate sample dilution buffer > Refer to Assay preparation
