Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).
Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively. It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).
DHR 123 probe is a READY TO USE probe suitable for: