KP06004 | DHR-123 (Dihydrorhodamine) Probe | Intracellular ROS Assay Kit
155.00€ – 310.00€ TAX excl.
Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).
Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively. It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).
DHR 123 probe is a READY TO USE probe suitable for:
Flow cytometer
Microscopy
Fluorimeter
Additional information
Sizes: 250, 500, and 1000 tests
Expiry date: 1 year
Storage: Room temperature
Assay time: 90 minutes
Protocol Booklet
Download the web version
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Safety Data Sheet (SDS)
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Publications
- Rodríguez S, García M, Fernández B, et al. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874
- Cepas V , Manig F, Mayo JC, et al. 2021. ” In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls”. Oxidative Medicine and Cellular Longevity. Volume 2021, Article ID 9912240. https://doi.org/10.1155/2021/9912240
FAQs
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