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KB03003 | Bradford Protein Quantification Assay Kit

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KB03003 | Bradford Protein Quantification Assay Kit

112.00193.00 TAX excl.

Bradford Protein Quantification Assay Kit is based on the direct binding of Coomassie brilliant blue dye to proteins at arginine, tryptophan, tyrosine, histidine, and phenylalanine residues. Specifically, the anionic dye binds to these residues producing an absorbance maximum at 595 nm.

The dye interacts with the protein via van der Waals forces between the hydrophobic regions of the protein and the nonpolar region of the dye and the electrostatic interaction between the negatively charged dye and positively charged amino groups of the protein. The formation of the dye-protein complex takes about two minutes and remains stable for an hour, so the procedure is very fast and the time for trial is not limiting. Additionally, the dye-protein complex has a high extinction coefficient, which makes it a very sensitive assay

Additional information

Sizes: 2000 and 4000 tests

Expiry date: 1 year

Storage: Room temperature and 4ºC

Assay time: 15 minutes

Protocol Booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

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Publications

  • Mayo JC, Hevia D, Quiros I, et al. 2017. “IGFBP3 and MAPK/ERK signaling mediates atonin-induced antitumor activity in prostate cancer.” Journal of Pineal Research 62 (1). https://doi.org/10.1111/jpi.12373
  • Cepas V , Manig F, Mayo JC, et al. 2021. ” In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls”. Oxidative Medicine and Cellular Longevity. Volume 2021, Article ID 9912240. https://doi.org/10.1155/2021/9912240

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