FAQs Archive - BQC - Bioquochem

Q: Alcian Blue Stain Kit | KH07001

BQC-Reference: KH07001 Publications : Ochiai J, Villanueva L, Niihara H, Niihara Y, & Oliva J. 2022. "Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets". Frontiers in Cell and Developmental Biology, 10. DOI: 10.3389/fcell.2022.873603

Q: Harris Hematoxylin-Eosin Stain Kit | KH07005

BQC-Reference: KH07005 Publications : Ochiai J, Niihara Y, and Oliva J. 2021. "Measurement of the adipose stem cells cell sheets transmittance". Bioengineering. 8,93. https://doi.org/10.3390/bioengineering8070093

Q: DHE Intracellular ROS Probe | KP06002

BQC-Reference: DHE probe Publications : Cepas V , Manig F, Mayo JC, et al. 2021. " In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls". Oxidative Medicine and Cellular Longevity. Volume 2021, Article ID 9912240. https://doi.org/10.1155/2021/9912240

Q: Multiprobe REDOX Assay Kit | KP06005

BQC-Reference: KP06005 Publications : <span data-sheets-value="{"1":2,"2":"Zhiyuan V, Le Gal K, El Zowalaty A. 2021. \"Antioxidants Promote Intestinal Tumor Progression in Mice\". Antioxidants 2021, 10(2), 241; https://doi.org/10.3390/antiox10020241"}" data-sheets-userformat="{"2":1325887,"3":{"1":0},"4":{"1":2,"2":16777215},"5":{"1":[{"1":2,"2":0,"5":{"1":0}},{"1":0,"2":0,"3":3},{"1":1,"2":0,"4":1}]},"6":{"1":[{"1":2,"2":0,"5":{"1":0}},{"1":0,"2":0,"3":3},{"1":1,"2":0,"4":1}]},"7":{"1":[{"1":2,"2":0,"5":{"1":0}},{"1":0,"2":0,"3":3},{"1":1,"2":0,"4":1}]},"8":{"1":[{"1":2,"2":0,"5":{"1":0}},{"1":0,"2":0,"3":3},{"1":1,"2":0,"4":1}]},"11":4,"12":0,"14":{"1":3,"3":1},"15":"Roboto","16":11,"21":0,"23":1}" data-sheets-textstyleruns="{"1":0}{"1":134,"2":{"2":{"1":2,"2":0},"9":0}}{"1":172,"2":{"2":{"1":2,"2":1136076},"9":1}}">Zhiyuan V, Le Gal K, El Zowalaty A. 2021. "Antioxidants Promote Intestinal Tumor Progression in Mice". Antioxidants, 10(2), 241. https://doi.org/10.3390/antiox10020241

Sample erratic values

Possible cause and recommended solutions: Samples contain interfering substances > Dilute sample further (if possible) Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use Samples not deproteinized > Use an appropriate deproteinization protocol Cells/Tissue samples not homogenized completely > Repeat the sample homogenization Inappropriate

adminBioq2022-10-18T13:38:28+02:00October 18th, 2022|FAQ table|0 Comments

Dispersion of standard and sample readings

Possible cause and recommended solutions: Pipetting errors > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique

adminBioq2022-10-18T13:38:59+02:00October 18th, 2022|FAQ table|0 Comments

Standard readings do not follow a linear pattern

Possible cause and recommended solutions: Pipetting errors in preparation of standards > Avoid pipetting small volumes (<5 µL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique Standard stock is at incorrect concentration > Always refer to dilutions described in Assay preparation Improperly thawed

adminBioq2022-10-18T13:40:07+02:00October 18th, 2022|FAQ table|0 Comments

Wells have color but there is no reading

Possible cause and recommended solutions: Plate read at incorrect wavelength > Check the wavelength used in the assay Incorrect microplate > Use the correct microplate for your application: UV/Vis: transparent Fluorescence: black wells/transparent bottom

adminBioq2022-10-18T13:55:23+02:00October 18th, 2022|FAQ table|0 Comments

Alcian Blue Stain Kit | KH07001

BQC-Reference: KH07001 Publications: Ochiai J, Villanueva L, Niihara H, Niihara Y, & Oliva J. 2022. "Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets". Frontiers in Cell and Developmental Biology, 10. DOI: 10.3389/fcell.2022.873603

adminBioq2022-09-01T10:06:25+02:00August 30th, 2022|Stain Line|0 Comments

Harris Hematoxylin-Eosin Stain Kit | KH07005

BQC-Reference: KH07005 Publications: Ochiai J, Niihara Y, and Oliva J. 2021. "Measurement of the adipose stem cells cell sheets transmittance". Bioengineering. 8,93. https://doi.org/10.3390/bioengineering8070093

adminBioq2022-09-01T10:07:14+02:00April 19th, 2022|Stain Line|0 Comments

DHE Intracellular ROS Probe | KP06002

BQC-Reference: DHE probe Publications: Cepas V , Manig F, Mayo JC, et al. 2021. " In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls". Oxidative Medicine and Cellular Longevity. Volume 2021, Article ID 9912240. https://doi.org/10.1155/2021/9912240

adminBioq2022-09-01T12:04:47+02:00April 19th, 2022|ROS Sensors|0 Comments

eBQC Natural Ingredients Device | eBQC0201

BQC-Reference: eBQC Natural ingredients | eBQC0201 Publications: Petrova P, Arsov A, Ivanov I, et al. 2021. "New Exopolysaccharides Produced by Bacillus licheniformis 24 Display Substrate-Dependent Content and Antioxidant Activity" Microorganisms 9, 2127. https://doi.org/10.3390/microorganisms9102127 del Mar Rivas-Chacón L, Yanes-Díaz J, de Lucas B, Riestra-Ayora J.I., et al. 2022. "Preventive Effect of Cocoa Flavonoids via Suppression of Oxidative

adminBioq2022-08-30T10:55:13+02:00January 10th, 2022|Devices|0 Comments

MDA-TBARs Assay Kit | KB03016

BQC-Reference: KB03016 Publications: Garcia J., Turiel D, Bettmer J, et al. 2020. "In vitro and in situ experiments to evaluate the biodistribution and cellular toxicity of ultrasmall iron oxide nanoparticles potentially used as oral iron supplements". Nanotoxicology,14(3):388-403. doi: 10.1080/17435390.2019.1710613. Chaklader MR, Siddik MAB, Fotedar R. 2020. "Total replacement of fishmeal with poultry by-product meal affected the

adminBioq2022-08-30T11:04:23+02:00March 29th, 2021|Biomolecule damage assays|0 Comments

Multiprobe REDOX Assay Kit | KP06005

BQC-Reference: KP06005 Publications: Zhiyuan V, Le Gal K, El Zowalaty A. 2021. "Antioxidants Promote Intestinal Tumor Progression in Mice". Antioxidants, 10(2), 241. https://doi.org/10.3390/antiox10020241

adminBioq2022-09-01T10:07:58+02:00March 29th, 2021|ROS Sensors|0 Comments

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