Protocols for some agri-food samples
How the BRS measures TAC based on electrochemistry?
Redox (reduction/oxidation) reactions are involved in the origin and consequences of oxidative stress. Electrochemistry is recognized as the best tool to study this type of reactions based on the electroactivity of most of the oxidants and antioxidants present in agri-food samples. BRS TAC results for agrifood analysis are expressed in terms of BRS Value.
What is TAC and why to assay this parameter?
Total antioxidant capacity (TAC) is a global measure of the non-enzymatic antioxidant activity that integrates the individual effect of all antioxidants in a given matrix, and their additive, synergistic or antagonistic interactions. TAC is considered as an important parameter to establish the redox state of agri-food samples. Due to the transient nature of ROS/RNS,
BRS features
ā¢ Download more information here The use of disposable strips and BRS Electrolyte is mandatory for agri-food samples ā¢ Shop BRS and accesories - Simple and fast analysis - Easy operation - No need of lab equipment - No modification of sample native conditions. Samples must only be mixed with a BRS
Sample erratic values
Possible cause and recommended solutions: Samples contain interfering substances > Dilute sample further (if possible) Inappropriately stored samples or samples used after multiple freeze-thaw cycles > Use fresh samples or store appropriately until use Samples not deproteinized > Use an appropriate deproteinization protocol Cells/Tissue samples not homogenized completely > Repeat the sample homogenization Inappropriate
Dispersion of standard and sample readings
Possible cause and recommended solutions: Pipetting errors > Avoid pipetting small volumes (<5 ĀµL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique
Standard readings do not follow a linear pattern
Possible cause and recommended solutions: Pipetting errors in preparation of standards > Avoid pipetting small volumes (<5 ĀµL) and be careful not to splash from well to well Air bubbles formed in well(s) > Use reverse pipetting technique Standard stock is at incorrect concentration > Always refer to dilutions described in Assay preparation Improperly thawed
Wells have color but there is no reading
Possible cause and recommended solutions: Plate read at incorrect wavelengthĀ > Check the wavelength used in the assay Incorrect microplate > Use the correct microplate for your application: UV/Vis: transparent Fluorescence: black wells/transparent bottom
Alcian Blue Stain Kit | KH07001
BQC-Reference: KH07001 Publications: Ochiai J, Villanueva L, Niihara H, Niihara Y, & Oliva J. 2022. "Posology and Serum-/Xeno-Free Engineered Adipose Stromal Cells Cell Sheets". Frontiers in Cell and Developmental Biology, 10. DOI: 10.3389/fcell.2022.873603
Harris Hematoxylin-Eosin Stain Kit | KH07005
BQC-Reference: KH07005 Publications: Ochiai J, Niihara Y, and Oliva J. 2021. "Measurement of the adipose stem cells cell sheets transmittance". Bioengineering. 8,93. https://doi.org/10.3390/bioengineering8070093
