Name: KF01007 | DPPH Assay Kit | Antioxidant Capacity
SKU: KF01007
Availability: InStock

KF01007 | DPPH Assay Kit | Antioxidant Capacity

Price range: 185.00€ through 460.00€ TAX excl.

BQC DPPH TAC Assay Kit provides a simple, fast, and reproducible method for assessing total antioxidant capacity (TAC) in a wide range of samples.

BQC DPPH TAC Assay Kit is based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. In this method, the DPPH free radical (DPPH•) which is a deep purple colored (λmax = 517 nm) stable organic nitrogen radical, is reduced by antioxidants to the colorless DPPH reduced form. Therefore, the absorbance decrease at 517 nm depends linearly on the antioxidant concentration.

The synthetic antioxidant Trolox (included in the kit) is used to standardize the sample TAC relative to Trolox (Trolox Equivalents Antioxidant Capacity, TEAC).

Test

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SKU: KF01007 Category: Total Antioxidant Capacity (TAC) Tags: Antioxidant, Antioxidant capacity, Colorimetric, DPPH, TAC

Additional information

Sizes: 100,200 and 400 tests

Expiry date: 1 year

Storage: -20ºC, 4ºC and room temperature

Assay time: 15 minutes

Reagents: Reagent A, Reagent B, and Standard (Trolox)

Necessary material: 96 well-plate spectrophotometer

If you need to adapt it for another form of the assay (for example cuvette), contact at support@bqcredox.com

Protocol Booklet

Download the web version

To access more information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Publications

2026

Al Srouji, S., El Kayal, W., Ismail, D., Nasreddine, L., Gali-Muhtasib, H., & Hwalla, N. (2026). Unveiling the antioxidant and anticancer potential of local mediterranean olive and fig extracts against breast cancer. BMC Complement Med Ther,26(116). https://doi.org/10.1186/s12906-026-05296-4
Nuzzo, D., Zupo, V., Esposito, R., Somma, E., Savarese, C., Carfagna, S., Salbitani, G., Panunzi, S., Pompa, M., De Gaetano, A., Girgenti, A., Palumbo, L., Picone, P., & Costantini, M. (2026). Effects of lighting technologies on the physiology of a marine diatom, Cocconeis scutellum parva (Bacillariophyceae): 2. Production of antioxidants and other bioactive compounds. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 302, 110451. https://doi.org/10.1016/j.cbpc.2026.110451
Romero-Villareal, K. M., Lobo-Farfan, I., Alcalá-Orozco, M., Carrascal, J. J., Anaya, B. J., Tirado, D. F., & Mantilla-Escalante, D. C. (2026). Biotechnological Transformation of Tropical Bee Pollen Through Enzymatic and Bacterial Processes: Impact on Composition and Antioxidant Activity. Applied Sciences, 16(3), 1471. https://doi.org/10.3390/app16031471

2025

Caballero-Gallardo, K., Quintero-Rincón, P., Stashenko, E. E., & Olivero-Verbel, J. (2025). Chemical composition and in vitro bioactivities of hydroalcoholic extracts from Turnera pumilea, Hyptis lantanifolia, and Ageratina popayanensis cultivated in Colombia. Scientific Reports, 15, 31189. https://doi.org/10.1038/s41598-025-12778-x
Girgenti, A., Contente, M. L., Buttacavoli, M., Palumbo, L., Naselli, F., Dallavalle, S., Borgonovo, G., Picone, P., Pinto, A., & Nuzzo, D. (2025). Protective effects of safranal against spike protein-induced mitochondrial dysfunction and inflammation in peripheral and central immune cells. Current Developments in Nutrition, Advance online publication, 107629. https://doi.org/10.1016/j.cdnut.2025.107629
Girgenti, A., Pasquale, P., Buttacavoli, M., Palumbo, L., Flores Naselli, E., Lo Presti, E., Pecora, D., Cipollina, C., Annunziata, F., Pinto, A., Tamborini, L., & Nuzzo, D. (2025). Mitochondria-targeted rosmarinic acid: Its role against oxidative damage. Biomedicine & Pharmacotherapy, 187, 118114. https://doi.org/10.1016/j.biopha.2025.118114
Griffin, S. A. (2025). Thesis (No. 145). Louisiana Tech University. https://digitalcommons.latech.edu/theses/145
Pecora, D. (2025). Development of flow processes for the efficient and sustainable preparation of high value chemicals (Doctoral dissertation, Università degli Studi di Milano). Dipartimento di Scienze Farmaceutiche. https://hdl.handle.net/2434/1166642
Pecora, D., Magni, A. M., Vicinanza, S., Annunziata, F., Princiotto, S., Donzella, S., Meroni, G., Martino, P. A., Basilico, N., Parapini, S., Conti, P., Borsari, C., & Tamborini, L. (2025). Two-step flow amidation of natural phenolic acids as antiradical and antimicrobial agents. Journal of Natural Products, 88(5), 1153–1159. https://doi.org/10.1021/acs.jnatprod.5c00131
Ponce-Mora, A., Gimeno-Mallench, L., Lavandera, J. L., Giebelhaus, R. T., Domenech-Bendaña, A., Locascio, A., Gutierrez-Rojas, I., Sauro, S., de la Mata, P., Nam, S. L., et al. (2025). Systematic characterization of antioxidant shielding capacity against oxidative stress of aerial part extracts of Anacardium occidentale. Antioxidants, 14(8), 935. https://doi.org/10.3390/antiox14080935
Wang, Z., Svyantek, A., & Miller, Z. (2025). Apple and grape waste pomace fermentation and co-ferment product chemistry. Fermentation, 11(3), 126. https://doi.org/10.3390/fermentation11030126
Wang, Z., Svyantek, A., Miller, Z., Davis, H., & Kapus, A. (2025). The influence of ultraviolet-C light pretreatment on blackcurrant (Ribes nigrum) quality during storage. Applied Sciences, 15(15), 8452. https://doi.org/10.3390/app15158452
Wang, Z., Svyantek, A., & Miller, Z. (2025). Effect of blue and red light-emitting diodes on haskap (Lonicera caerulea) postharvest storage. Agricultural Production, Processing and Storage, 1(3). https://doi.org/10.1007/s44462-025-00006-8

2024

Kyriakou, S., Demosthenous, N., Amery, T., Stewart, K. J., Winyard, P. G., Franco, R., Pappa, A., & Panayiotidis, M. I. (2024). Naturally derived phenethyl isothiocyanate modulates induction of oxidative stress via its N-acetylated cysteine conjugated form in malignant melanoma. Antioxidants, 13(1), 82. https://doi.org/10.3390/antiox13010082
Marcin, N., Thesnor, V., Duvauchelle, V., Ponce-Mora, A., Gimeno-Mallench, L., Narayanin-Richenapin, S., Brelle, L., Bejarano, E., Yacou, C., Sylvestre, M., Onésippe-Potiron, C., Meffre, P., Benfodda, Z., & Cebrian-Torrejon, G. (2024). Characterization of alginates of Sargassum from the Archipelago of Guadeloupe. Separations, 11(8), 226. https://doi.org/10.3390/separations11080226
Vicinanza, S., Mombelli, L., Annunziata, F., Donzella, S., Contente, M. L., Borsari, C., Conti, P., Meroni, G., Molinari, F., Martino, P. A., Pinto, A., & Tamborini, L. (2024). Chemo-enzymatic flow synthesis of nature-inspired phenolic carbonates and carbamates as antiradical and antimicrobial agents. Sustainable Chemistry and Pharmacy, 39, 101542. https://doi.org/10.1016/j.scp.2024.101542
Wang, Z., Svyantek, A., Miller, Z., & Watrelot, A. A. (2024). Fermentation process effects on fermented McIntosh apple ciders. Fermentation, 10(3), 115. https://doi.org/10.3390/fermentation10030115

2023

Caballero-Gallardo, K., Alvarez-Ortega, N., & Olivero-Verbel, J. (2023). Cytotoxicity of nine medicinal plants from San Basilio de Palenque (Colombia) on HepG2 cells. Plants, 12(14), 2686. https://doi.org/10.3390/plants12142686
Hammoud, M., Rajha, H. N., Chokr, A., Safi, C., Van Den Broek, L. A. M., Van VanErven, G., Maroun, R. G., Debs, E., Rammal, H., & Louka, N. (2023). Chemical composition and biological activities of Centranthus longiflorus stems extracts recovered using Ired-Irrad®, an innovative infrared technology, compared to water bath and ultrasound. Life, 13(6), 1288. https://doi.org/10.3390/life13061288
Rendueles, E., González, N., Fernández, J. R., Álvarez, V., Menéndez, R., & Blanco, D. (2023). Biochemical profile and antioxidant properties of propolis from northern Spain. Foods, 12(23), 4337. https://doi.org/10.3390/foods12234337
Simon, A., Mazhar, S., Khokhlova, E., Leeuwendaal, N., Phipps, C., Deaton, J., Rea, K., & Colom, J. (2023). Solarplast®—An enzymatically treated spinach extract. Plants, 12, 2678. https://doi.org/10.3390/plants12142678

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KF01007 | DPPH Assay Kit | Antioxidant Capacity