KF01001 | DMPD Assay Kit | Antioxidant Capacity
180.00€ – 430.00€ TAX excl.
A solution of DMPD at an acidic pH, in the presence of a suitable oxidant solution, can form a stable and colored radical cation (DMPD•+) that shows maximum absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce discoloration of the solution which is proportional to their amount. This reaction is rapid and the endpoint, which is stable, corresponds to a measure of the antioxidative efficiency.
This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms.
Additional information
Sizes: 100,200 and 400 tests
Expiry date: 1 year
Storage: -20ºC, 4ºC and room temperature
Assay time: 30 minutes
Reagents: Reagent A, Reagent B, Reagent C, Reagent D, and Standard (Trolox)
Necessary material: 96 well-plate spectrophotometer
If you need to adapt it for another form of the assay (for example cuvette), contact at info@bioquochem.com
Protocol Booklet
Download the web version
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Safety Data Sheet (SDS)
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Publications
- Rydlová L, Tesařová E, Valentová K, and Křen V. 2017. “Enzymatic modifications of bioactive flavonoids diplomová.” Univerzita Karlova V Praze.
- Valentová K, Káňová K, Di Meo F, Pelantová H, et al. 2017. “Chemoenzymatic preparation and biophysical properties of sulfated quercetin metabolites.” International Journal of Molecular Sciences 18 (11): 1–17. https://doi.org/10.3390/ijms18112231
- Valentová K, Purchartová K, Rydlová L, et al. 2018. “Sulfated metabolites of flavonolignans and 2,3-Dehydroflavonolignans: preparation and properties.” International Journal of Molecular Sciences 19 (2349). https://doi.org/10.3390/ijms19082349
- Biedermann D, Moravcová V, Kateřina V, et al. 2019. “Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative.” Phytochemistry Letters 30 (January): 14–20. https://doi.org/10.1016/j.phytol.2019.01.006
- Sanchiz A, Pedrosa M, Guillamón E, et al. 2019. “Influence of Boiling and Autoclave Processing on the Phenolic Content, Antioxidant Activity and Functional Properties of Pistachio, Cashew and Chestnut Flours.” Lwt – Food Science and Technology 105: 250–56. https://doi.org/10.1016/j.lwt.2019.02.035.
- Káňová K, Petrásková L, Pelantová H, et al. 2020. “Sulfated metabolites of luteolin, myricetin, and ampelopsin: Chemoenzymatic preparation and biophysical properties”. Journal of Agricultural and Food Chemistry, 68(40), 11197–11206. https://doi:10.1021/acs.jafc.0c03997
FAQs
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