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KP06004 | DHR-123 (Dihydrorhodamine) | Intracellular ROS assay

147.00295.00 TAX excl.

Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).

Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively. It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).

DHR 123 probe is a READY TO USE probe suitable for:
Flow cytometer
Microscopy
Fluorimeter

SKU: KP06004Categories: Tags: , ,
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Description

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Test

250, 500, 1000

Publications

  • Rodríguez-Menéndez, Sara, Montserrat García, Beatriz Fernández, Lydia Álvarez, Andrés Fernández-Vega-Cueto, Miguel Coca-Prados, Rosario Pereiro, and Héctor González-Iglesias. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874

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