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KP06004 | DHR-123 (Dihydrorhodamine) | Intracellular ROS assay

147.00295.00 TAX excl.

Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123).

Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively. It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).

DHR 123 probe is a READY TO USE probe suitable for:
Flow cytometer
Microscopy
Fluorimeter

SKU: KP06004Categories: Tags: , ,
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Additional information

Sizes: 250, 500, and 1000 tests

Expiry date: 1 year

Storage: Room temperature

Assay time: 90 minutes

Protocol booklet

To access this information, please complete the fields. As soon as possible, the documentation will be sent to your email.

Safety Data Sheet (SDS)

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Publications

  • Rodríguez S, García M, Fernández B, et al. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874
  • Cepas V , Manig F, Mayo JC, et al. 2021. ” In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls”. Oxidative Medicine and Cellular Longevity. Volume 2021, Article ID 9912240. https://doi.org/10.1155/2021/9912240

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