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KP06003 | DCFH-DA probe | Intracellular ROS assay

179.00322.00 TAX excl.

2’-7’dichlorofluorescin diacetate (DCFH-DA) is a cell-permeant reagent fluorogenic dye that measures hydroxyl, peroxyl, and other ROS activity in the cell. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF). Then, DCF can be detected by fluorimetry, flow cytometry or fluorescence microscopy, with maximum excitation and emission spectra of 495 nm and 529 nm, respectively.

DCFH-DA probe is a READY TO USE probe suitable for:
Flow cytometer
Microscopy
Fluorimeter

SKU: KP06003Categories: Tags: , ,
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Additional information

Test

250, 500, 1000

Publications

  • Garcia-Fernandez, Jenifer, Daniel Turiel, Jorg Bettmer, Norbert Jakubowski, Ulrich Panne, Lorenzo Rivas García, Juan Llopis, Cristina Sánchez González, and Maria Montes-Bayón. 2020. “In Vitro and in Situ Experiments to Evaluate the Biodistribution and Cellular Toxicity of Ultrasmall Iron Oxide Nanoparticles Potentially Used as Oral Iron Supplements.” Nanotoxicology 14 (3): 388–403. https://doi.org/10.1080/17435390.2019.1710613.
  • Gonzalez-Menendez, Pedro, David Hevia, Rebeca Alonso-Arias, Alejandro Alvarez-Artime, Aida Rodriguez-Garcia, Sandrina Kinet, Ivan Gonzalez-Pola, Naomi Taylor, Juan C. Mayo, and Rosa M. Sainz. 2018. “GLUT1 Protects Prostate Cancer Cells from Glucose Deprivation-Induced Oxidative Stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017
  • Rodríguez-Menéndez, Sara, Montserrat García, Beatriz Fernández, Lydia Álvarez, Andrés Fernández-Vega-Cueto, Miguel Coca-Prados, Rosario Pereiro, and Héctor González-Iglesias. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874
  • Gonzalez-Menendez, Pedro, David Hevia, Rebeca Alonso-Arias, Alejandro Alvarez-Artime, Aida Rodriguez-Garcia, Sandrina Kinet, Ivan Gonzalez-Pola, Naomi Taylor, Juan C. Mayo, and Rosa M. Sainz. 2018. “GLUT1 Protects Prostate Cancer Cells from Glucose Deprivation-Induced Oxidative Stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017.
  • Rodríguez-Menéndez, Sara, Montserrat García, Beatriz Fernández, Lydia Álvarez, Andrés Fernández-Vega-Cueto, Miguel Coca-Prados, Rosario Pereiro, and Héctor González-Iglesias. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874.
  • Garcia-Fernandez, J., Turiel, D., Bettmer, J., Jakubowski, N., Panne, U., Rivas García, L., … Montes-Bayón, M. (2020). In vitro and in situ experiments to evaluate the biodistribution and cellular toxicity of ultrasmall iron oxide nanoparticles potentially used as oral iron supplements. Nanotoxicology, 1–16.

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