KP06003 | DCFH-DA Probe | Intracellular ROS Assay Kit

199.00356.00 TAX excl.

2’-7’dichlorofluorescin diacetate (DCFH-DA) is a cell-permeant reagent fluorogenic dye that measures hydroxyl, peroxyl, and other ROS activity in the cell. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF). Then, DCF can be detected by fluorimetry, flow cytometry or fluorescence microscopy, with maximum excitation and emission spectra of 495 nm and 529 nm, respectively.

DCFH-DA probe is a READY TO USE probe suitable for:
Flow cytometer
Microscopy
Fluorimeter

SKU: KP06003Categories: Tags: , ,

Additional information

Sizes: 250, 500 and 1000 tests

Expiry date: 1 year

Storage: -20 ºC

Assay time: 90 minutes

Reagents: Reagent A, Reagent B, and Reagent C

Protocol Booklet

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Safety Data Sheet (SDS)

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Publications

  • Gonzalez P, Hevia D, Alonso R, et al. 2018. “GLUT1 protects prostate cancer cells from glucose deprivation-induced oxidative stress.” Redox Biology 17 (April): 112–27. https://doi.org/10.1016/j.redox.2018.03.017
  • Rodríguez S, García M, Fernández B, et al. 2018. “The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells.” Nutrients 10 (12). https://doi.org/10.3390/nu10121874
  • Garcia J, Turiel D, Bettmer J, et al. 2020. “In Vitro and in Situ Experiments to Evaluate the distribution and Cellular Toxicity of Ultrasmall Iron Oxide Nanoparticles Potentially Used as Oral Iron Supplements.” Nanotoxicology 14 (3): 388–403. https://doi.org/10.1080/17435390.2019.1710613
  • Bajes, Hana, Sawsan Oran, and Yasser Bustanji. “Phytochemical analysis, In vitro Assessment of antioxidant properties and cytotoxic potential of Thymus capitatus essential oil.” Research Journal of Pharmacy and Technology 16.3 (2023): 1100-1108
  • Tawfeeq S. H., & Kakey, I. S. 2022. “Melatonin’s Antioxidative Characteristic in Human Aging Retinal Pigment Epithelial Cells”. Indian Journal of Pharmaceutical Sciences, 64-70.DOI: 10.36468/pharmaceutical-sciences.spl.492
  • Coveñas Vilchez, Carmen Lucia. “Evaluación del efecto del resveratrol en la criopreservación de células madre espermatogoniales de Vicugna pacos (Linnaeus, 1758)“alpaca”.” (2023)

FAQs

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