2’-7’dichlorofluorescin diacetate (DCFH-DA) is a cell-permeant reagent fluorogenic dye that measures hydroxyl, peroxyl, and other ROS activity in the cell. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF) with maximum excitation and emission spectra of 495 nm and 529 nm, respectively.
KP06003 | DCFH-DA Probe | Intracellular ROS Assay Kit
Price range: 219.00€ through 393.00€ TAX excl.
Additional information
Sizes: 250, 500 and 1000 tests
Expiry date: 1 year
Storage: -20 ºC
Assay time: 90 minutes
Protocol Booklet
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Safety Data Sheet (SDS)
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Publications
2025
- AlAbduljader, H., AlSaeed, H., Alrabeea, A., Sulaiman, A., Haider, M. J. A., Al-Mulla, F., Ahmad, R., & Al-Rashed, F. (2025). Eicosapentaenoic Acid (EPA) Alleviates LPS-Induced Oxidative Stress via the PPARα–NF-κB Axis. Oxidative Medicine and Cellular Longevity, Article ID 3509596. https://doi.org/10.1155/omcl/3509596
2023
- Bajes, H., Oran, S., & Yasser, B. (2023). Phytochemical Analysis, In vitro Assessment of Antioxidant Properties and Cytotoxic Potential of Thymus capitatus Essential Oil. Research Journal Of Pharmacy And Technology, 1100–1108. https://doi.org/10.52711/0974-360x.2023.00183
- Coveñas Vilchez, C. L. (2023). Evaluación del efecto del resveratrol en la criopreservación de células madre espermatogoniales de Vicugna pacos (Linnaeus, 1758) “alpaca”. https://hdl.handle.net/20.500.12672/20541
2022
- Tawfeeq, G. S., & Kakey, I. S. (2022). Melatonin’s Antioxidative Characteristic in Human Aging Retinal Pigment Epithelial Cells. Indian Journal Of Pharmaceutical Sciences, 84(S3). https://doi.org/10.36468/pharmaceutical-sciences.spl.492
2020
- Garcia-Fernandez, J., Turiel, D., Bettmer, J., Jakubowski, N., Panne, U., García, L. R., Llopis, J., González, C. S., & Montes-Bayón, M. (2020). In vitro and in situ experiments to evaluate the biodistribution and cellular toxicity of ultrasmall iron oxide nanoparticles potentially used as oral iron supplements. Nanotoxicology, 14(3), 388–403. https://doi.org/10.1080/17435390.2019.1710613
2018
- Gonzalez-Menendez, P., Hevia, D., Alonso-Arias, R., Alvarez-Artime, A., Rodriguez-Garcia, A., Kinet, S., Gonzalez-Pola, I., Taylor, N., Mayo, J. C., & Sainz, R. M. (2018). GLUT1 protects prostate cancer cells from glucose deprivation-induced oxidative stress. Redox Biology, 17, 112–127. https://doi.org/10.1016/j.redox.2018.03.017
- Rodríguez-Menéndez, S., García, M., Fernández, B., Álvarez, L., Fernández-Vega-Cueto, A., Coca-Prados, M., Pereiro, R., & González-Iglesias, H. (2018). The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients, 10(12), 1874. https://doi.org/10.3390/nu10121874
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