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KC04012 | Caspase-9 Fluorimetric Assay Kit

268.00535.00 TAX excl.

Caspase-9 Fluorimetric Assay Kit provides a simple procedure for assaying the activity of caspases that recognize the sequence LEHD. Consequently, the user can detect the fluorophore 7-amino-4-trifluoromethylcoumarin (AFC), after cleavage from the labeled substrate LEHD-AFC. The excitation and emission wavelength range for monitoring AFC in a spectrofluorometer are 400 nm and 480-520 nm, respectively. Comparison of AFC fluorescence from an apoptotic sample vs AFC fluorescence of control allows determination of the fold increase in caspase-9 activity.

There are two classes of caspases that involve apoptosis; initiators and effectors. Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. As a consequence, activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. As a resume, initiator caspases (caspase 2, 8, 9, and 10) initiate the apoptosis signal and the executioner caspases (caspase 3, 6, and 7) carry out the mass proteolysis that leads to apoptosis.

SKU: KC04012Categories: Tags: ,

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