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KC04011 | Caspase-9 Colorimetric Assay Kit

268.00535.00 TAX excl.

Caspase-9 Colorimetric Assay Kit provides a simple procedure for assaying the activity of caspases that recognize the sequence LEHD. Hence, the user can detect the chromophore p-nitroanilide (pNA), after cleavage from the labeled substrate LEHD-pNA. The pNA can be quantified using a spectrophotometer or a microplate reader at 405 nm.  Comparison pNA absorbance from an apoptotic sample vs absorbance of control allows determination of the fold increase in caspase-9 activity

There are two classes of caspases that involve apoptosis; initiators and effectors. Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. As a consequence, activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. As a resume, initiator caspases (caspase 2, 8, 9, and 10) initiate the apoptosis signal and the executioner caspases (caspase 3, 6, and 7) carry out the mass proteolysis that leads to apoptosis.

SKU: KC04011Categories: Tags: ,
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