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KB03005 | BCA Protein Quantification Assay

145.00230.00 TAX excl.

The BCA Protein Assay is a detergent-compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by the reduction of the Cu2+ to Cu+.

The purple-coloured reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex displays a strong absorbance at 562 nm.

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