BQC ABTS Assay Kit is a quick, simple, and reproducible assay to determine total antioxidant capacity (TAC) in a wide variety of samples.
This Kit is based on the interaction between antioxidants and the pre-formed green–blue stable radical cationic chromophore, 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate, ABTS•+). In the presence of antioxidants, the oxidized ABTS•+ radical is reduced to ABTS resulting in a discoloration of the solution, measured by the decrease in absorbance at 734 nm. Antioxidants scavenge ABTS•+ radical cation in a concentration dependent manner.
In this assay, Trolox or Vitamin C can be used to standardize the sample TAC relative to Trolox (Trolox Equivalents Antioxidant Capacity, TEAC) or Vitamin C (Vitamin C Equivalents Antioxidant Capacity, CEAC).
